Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Cellular Behaviour in Root Meristem Cells of Zinnia Elegans in Vivo and in Tissue Culture Systems

Rosna Mat Taha, and Rashidi Othman, (2002) Cellular Behaviour in Root Meristem Cells of Zinnia Elegans in Vivo and in Tissue Culture Systems. Malaysian Journal of Science, 21 (1 & 2). pp. 17-20. ISSN 1394-3065

Full text not available from this repository.

Official URL: http://www.um.edu.my/FS

Affiliations

University of Malaya, Faculty of Science, Institute of Biological Sciences, Malaysia

Abstract

Zinnia elegans is an attractive ornamental plant imported from overseas. Tissue culture of this species is beneficial for mass propagation, especially for commercial purposes. In this paper, we report on cellular studies of this species which is rarely done. Cellular parameters such as Mitotic Index (MI), chromosomes counts, ploidy level/nuclear DNA content, cell doubling time (Cdt) and measurement of mean cell and nuclear areas were recorded from root meristem cells grown in vivo and roots cultured in vitro. In this study, we have found that in vitro root under short term culture, showed an increase in the MI value, the chromosome number remained stable, the mean cell and nuclear areas decreased, the polyploid cells increased slightly, more cells entering S phase of the cell cycle and the duration of Cdt shortened in vitro. These behaviour are consistent with the readiness of this species to regenerate in vitro and the long term culture confirmed this observation and the regenerants obtained were true to type.

Item Type:Journal
Keywords:tissue culture, cellular behaviour Zinnia elegans, cell cycle
Subjects:Q Science, Computer Science
ID Code:1692

1. Abdullah H. (1998). Cellular activities and flowering of Petunia Hybrida Vilm. Var. ‘White Innocence’ in vitro. M. Sc. Thesis. University of Malaya.

2. Abu Shah N. and Rosna Mat Taha. (1994). Studies on cellular Behaviour in roots of Psophocarpus tetragonolobus (L.) DC. In vivo and in vitro. Asia Pac. J. Mol. Biol. & Biotech. 2: 316-326.

3. Clowes, F.A.L. (1961). Duration of the mitotic cycle in a meristem. J. of Experimental Botany 12: 283-293.

4. Evans, H.J., Neary, G.J. and Tonkinson, S.M. (1957). The use of colchicine as an indicator of mitotic rate in broad bean root meristems. J. of Genetics 55: 487-502.

5. Jordan, E. G., Hide, P., and Phillips, R. (1987). Pattems of nuclear and nucleolar growth in synchronously dividing explants from tubers of Helianthus tuberosus L. Ann. Bot. 59: 525-532.

6. Larkin, P.J. and Scowcroft, W.R. (1981). Somaclonal variation- a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197-214.

7. Minocha, S.C. (1979). Abscisic acid promotion of cell division and DNA synthesis in Jerusalem artichoke Helianthus tuberosus tuber tissue cultured in vitro. Zeitschift für Pflanzenphysiologie 92: 327-340.

8. Partenen, C. R. (1963). Plant tissue culture in relation to developmental cytology. Int. Rev. Cytol 15: 215-243.

9. Wright, J. W. (1976). Introduction to forest genetics. Academic Press, New York pp. 201-202.

10. Yeoman, M.M. and Mitchell, J.P. (1970). Changes accompanying the addition of 2, 4-D to excised Jerusalem artichoke tuber tissue. Ann. Bot. 34: 799-810.

11. Yeoman, M. M., Dyer, A.F., Robertson, A.I. (1965). Growth and differentiation of plant tissue cultures, 1. Changes accompanying the growth of explants from Helianthus tuberosus tubers. Ann. Bot. 29: 265-275.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.