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Morphogenesis of Cymbidium atropurpureum In-Vitro

Gayathery Subramaniam, and Rosna Mat Taha, (2003) Morphogenesis of Cymbidium atropurpureum In-Vitro. Malaysian Journal of Science, 22 (1). pp. 1-5. ISSN 1394-3065

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Official URL: http://www.um.edu.my/FS/Index.htm

Affiliations

University of Malaya, Faculty of Science, Institute of Biological Sciences, Malaysia

Abstract

Shoot tips excised from the orchid, $Cymbidium$ $atropurpureum$, produced protocorm-like bodies (PLB) on hormone-free Vacin and Went medium and hormone supplemented Vacin and Went (VW) medium. Explants showed the highest PLB formation (63.33%) on the medium supplemented with 5.0 mg/l of $\alpha$-naphthaleneacetic acid (NAA). The PLBs obtained were regenerated on hormone-free Murashige and Skoog (MS) medium. Besides PLB formation, callus formation from shoot tips was also observed in this study. Pseudobulb, rhizome and root explants produced callus on 1/2 strength MS mdium supplemented with 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l of thiadiazuron. The callus was maintained by sub-culturing in the same medium. Further experiments to determine if the callus obtained is organogenic or embryogenic is in progress.

Item Type:Journal
Keywords:Protocorm-like bodies, shoot tip, morphogenesis, regeneration, callus, pseudobulb.
Subjects:Q Science
ID Code:1730

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