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Mutational analysis of p53 and RB2/p130 genes in Malaysian nasopharyngeal carcinoma samples: a preliminary report

Hoe, Susan Ling Ling, and Sam, CK, (2006) Mutational analysis of p53 and RB2/p130 genes in Malaysian nasopharyngeal carcinoma samples: a preliminary report. Malaysian Journal of Pathology, 28 (1). pp. 35-39. ISSN 0126-8635

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Official URL: http://www.mjpath.org.my

Affiliations

Institute for Medical Research. Cancer Research Centre
University of Malaya. Institute of Biological Sciences

Abstract

This study reports the results of mutation detection of tumour suppressor genes, p53 and RB2/p130 genes in Malaysian nasopharyngeal carcinoma (NPC) studied by PCR-CSGE analysis and direct DNA sequencing method. Frequent sites of mutation in both genes (exons 5-8 of p53 and exons 19-21 of RB2/p130) were examined. Thirty-six NPC blood samples and three NPC cell lines were investigated for the presence of mutations. No mutation of p53 and RB2/p130 genes was identified in any of the blood samples. Nonetheless, there was an identical G → C nucleotide change at codon 280 of p53 gene in all the cell lines. A larger study that includes biopsy tissues should be carried out to provide a more in-depth look into the pathogenesis of NPC in Malaysia.

Item Type:Journal
Keywords:PCR-CSGE assay, mutation, nasopharyngeal carcinoma
Subjects:R Medicine
ID Code:1778

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