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Antigenic cell associated dengue 2 virus proteins detected in vitro using dengue fever patients sera

Sazaly Abu Bakar, and Azila Azmi, and Suzana Misbah, and Chang, Li Yen, (2002) Antigenic cell associated dengue 2 virus proteins detected in vitro using dengue fever patients sera. Malaysian Journal of Pathology, 24 (1). pp. 29-36. ISSN 0126-8635

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Official URL: http://www.mjpath.org.my/past_issue/MJP2002.1/Antigenic%20cell%20associated%20dengue%202%20virus%20proteins.pdf

Affiliations

University of Malaya. Faculty of Medicine. Dept. of Medical Microbiology
University of Malaya. Faculty of Medicine. Dept. of Medical Microbiology
University of Malaya. Faculty of Medicine. Dept. of Medical Microbiology
University of Malaya. Faculty of Medicine. Dept. of Medical Microbiology

Abstract

At least three major antigenic dengue 2 virus proteins were recognized by pooled dengue fever patients' sera in infected Aedes albopictus (C6136) mosquito cells. Dengue virus envelope (E), premembrane(PrM) and non-structural protein 1 (NS1) dimer were detected beginning on day 3 postinfection in both the cell membrane and cytosolic fractions. Using the patients' sera, the presence of antigenic intermediate core protein (C)-PrM and NS1-non-structural protein 2a (NS2a) in the cytoplasmic fraction of dengue 2 virus infected cells was revealed. The presence of a - 92 and - 84 kDa NS 1 dimer in the membrane (NS lm) and cytosolic (NS lc) fractions of C6136 cells, respectively,
was also recognized. Using individual patient's serum, it was further confirmed that all patients' sera contained antibodies that specifically recognized E, NS1 and PrM present in the dengue 2 virusinfected cell membrane fractions, suggesting that these glycosylated virus proteins were the main antigenic proteins recognized in vivo. Detection of dengue 2 virus C antibody in some patients further suggested that C could be antigenic if presented in vivo.

Item Type:Journal
Additional Information:The study was funded in part by the Ministry of Science, Technology and Environment, Malaysia, IRPA grants #06-02-03-0303/0304.
Keywords:antigenic, dengue fever, dengue virus, mosquito, patients
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:1821

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