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Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR)

Latifah, I., and Teoh, K.Y., and Rahmah M., and Wan, K.L., and Normaznah Y., and Rohani A., (2005) Detection of giardine gene in local isolates of Giardia duodenalis by polymerase chain reaction (PCR). Malaysian Journal of Pathology, 27 (2). pp. 83-89. ISSN 0126-8635

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Official URL: http://www.mjpath.org.my/past_issue/MJP2005.2/02Giardine.pdf

Affiliations

Institute for Medical Research, Kuala Lumpur, Biotechnology Unit
Institute for Medical Research, Kuala Lumpur, Biotechnology Unit
Universiti Kebangsaanb Malaysia, Centre for Gene Analysis and Technology
Universiti Kebangsaanb Malaysia, Centre for Gene Analysis and Technology
Institute for Medical Research, Kuala Lumpur, Biotechnology Unit

Abstract

Giardia duodenalis is an intestinal parasite that causes diarrhoea and malabsorption in children. The parasite also infects AIDS patients with a weak immune system. A study was carried out on six local isolates of Giardia duodenalis (110, 7304, 6304, M007, 2002 and 6307) from faeces of Orang Asli patients admitted to the Gombak Hospital. WB, a reference pathogenic strain from human and G. muris from a wild mouse, were commercially obtained from the American Type Culture Collection (ATCC). All the isolates were cultured axenically in TYI-S-33 medium. Two sets of primers were used for the techniques: primers LP1 and RP1 and primers LP2 and RP2. The sets of primers amplified giardine gene of 171 bp and 218 bp in sizes respectively. The study showed that the two sets of primers could detect G. duodenalis to the genus and species level specifically.

Item Type:Journal
Additional Information:This project was financially supported by IRPA grant 06-05-01-0151.
Keywords:Giardiasis, Giardia duodenalis, giardine gene, polymerase chain reaction
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:1825

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