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Heterogeneous t(4;11) fusion transcripts in two infants with acute lymphoblastic leukemia

Gill, Harvinder Kaur, and Ten, Sew Keoh, and Dhaliwal, Jasbir Singh, and Moore, Sarah, and Roshida Hassan, and Faraiza Abdul Karim, and Zubaidah Zakaria, and Shahnaz Murad, and Mahfuzah Mohamad, and Hishamshah Mohamad Ibrahim, and Eni Juraida Abdul Rahman, (2004) Heterogeneous t(4;11) fusion transcripts in two infants with acute lymphoblastic leukemia. Malaysian Journal of Pathology, 26 (2). pp. 105-110. ISSN 0126-8635

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Official URL: http://www.mjpath.org.my/past_issue/MJP2004.2/04%20Heterogeneous.pdf

Affiliations

Institute for Medical Research, Kuala Lumpur. Allergy & Immunology Research Centre
Institute for Medical Reseaech, Kuala Lumpur. Cancer Research Centre
Institute for Medical Research, Kuala Lumpur. Allergy & Immunology Research Centre
Institute of Medical & Veterinary Science, Adelaide, South Australia
Kuala Lumpur Hospital, Pathology Department
National Blood Centre, Kuala Lumpur
Institute for Medical Reseaech, Kuala Lumpur. Cancer Research Centre
Institute for Medical Research, Kuala Lumpur. Allergy & Immunology Research Centre
Kuala Lumpur Hospital, Paediatric Institute
Kuala Lumpur Hospital, Paediatric Institute
Kuala Lumpur Hospital, Paediatric Institute

Abstract

An RT-PCR assay detected the t(4;11) translocation in two infants with acute lymphoblastic leukemia (ALL). Case P76 was a 10-month-old, female infant, who presented with a WBC of 137.4 x 109/l and a pre-pre-B ALL immunophenotype. Case P120 was 6-month-old female infant, with a WBC > 615 x 109/l and a pre-pre-B ALL immunophenotype. RT-PCR of cDNA from both these cases generated a 656 bp and a 542 bp respectively, which sequencing confirmed as t(4;11) fusion transcripts. The primers and conditions selected for this assay are compatible with a one-step multiplex PCR for the main translocations in childhood ALL.

Item Type:Journal
Keywords:RT-PCR assay, acute lymphoblastic leukemia
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:1842

1. DiMartino JF, Cleary ML. MLL rearrangements in haematological malignancies: lessons from clinical and biological studies. Br J Haematol 1999; 106:614-26.

2. Thirman MJ, Gill HJ, Burnett RJ, Mbangkollo D, McCabe NR, Kobayashi H et al. Rearrangement of the MLL gene in acute lymphoblastic leukemias with 11q23 chromosomal translocations. N Engl J Med 1992; 329:909-14.

3. Secker-Walker LM on behalf of the European Workshop participants. General report on the European Union concerted action workshop on 11q23, London, UK, May 1997. Leukemia 1998; 12:776-8.

4. Butler LH, Slany R, Cui X, Cleary ML, Mason DY. The HRX proto-oncogene product is widely expressed in human tissues and localizes to nuclear structures. Blood 1997; 89:3361-70.

5. Rasio D, Schichman SA, Negrini M, Canaani E, Croce CM. Complete exon structure of the ALL1 gene. Cancer Res 1996; 56:1766-9.

6. Nilson I, Lochner K, Seigler G, Greil J, Beck JD,Fey GH, et al. Exon/intron structure of the ALL-1 (MLL) gene involved in translocations to chromosomal region 11q23 and acute leukemias. Br J Haematol 1996; 93:966-72.

7. Li Q, Frestedt JL, Kersey JH. AF4 encodes a ubiquitous protein that in both native and MLL-AF4 fusion types localizes to subnuclear compartments. Blood 1998; 92: 3841-7.

8. Nilson I, Reichel M, Ennas MG, Greim R, Knorr C, Siegler G, et al. Exon /Intron structure of the human AF-4 gene, a member of the AF-4/ LAF-4/FMR-2 gene family coding for a nuclear protein with structural alterations in acute leukemia. Br J Haematol 1997; 98:157-69.

9. Morrisey JJ, Raney S, Cleary ML. The FEL (AF-4) protein donates transcriptional activation sequences to HRX-FEL fusion proteins in leukemias containing t(4;11)(q21;q23) chromosomal translocations. Leuk Res 1997; 21:911-7.

10. Isnard P, Core N, Nacquet P, Djabali M. Altered lymphoid development in mice deficient for the mAF4 proto-oncogene. Blood 2000; 96:705-10.

11. Hess JL, Yu BD, Li B, Hanson R, Korsmeyer SJ. Defects in yolk sac hematopoiesis in MLL-null embryos. Blood 1997; 90:1799-1806.

12. Felix CA & Lange BL. Leukemia in Infants. Oncologist 1999; 4:225-240.

13. Gabert J, Beillard E, van der Velden VHJ, Bi W, Grimwade D, Pallisgaard N, et al. Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - A Europe Against cancer Program. Leukemia 2003; 17:2318-57.

14. Johansson B, Moorman AV, Haas OA, Watmore AE, Cheung KL, Swanton S, et al., on behalf of the European 11q23 Workshop participants. Hematologic malignancies with t(4;11)(q21;q23)-a cytogenetic, morphologic, immunophenotypic and clinical study of 183 cases. Leukemia 1998; 12:779-87.

15. Gill HK, Ten SK, Dhaliwal JS, Moore S, Hassan R, Abdul Karim F et al. TEL-AML1 frequency in multi-ethnic Malaysian pediatric acute lymphoblastic leuekmia. Cancer Genet Cytogenet. In press 2005.

16. Cerveira N, Ferreira S, Doria S, Veiga I, Ferreira F, Mariz HM, et al. Detection of prognostic significant translocations in childhood acute lymphoblastic leukemia by one-step multiplex reverse transcription polymerase chain reaction. Br J Haematol 2000; 109:638-40.

17. Poteat HT, Sklar J. A simplified polymerase chain reaction assay for detection of chromosomal translocations in hematologic malignancies. Diagn Mol Path 1997; 6:3-9.

18. Chessells JM, Harrison CJ, Watson SL, Vora AJ, Richards SM for the Medical Research Council Working Party on Childhood Leukemia. Treatment of infants with lymphoblastic leukemia: results of the UK Infant Protocols 1987-1999. Br J Haematol 2002; 117:306-14.

19. Downing JR, Head DR, Raimondi SC, Carroll AJ, Curcio-Brint AM, Motroni TA, et al. The der(11)-encoded MLL/AF-4 fusion transcript is consistently detected in t(4;11)(q21;q23)-containing acute lymphoblassic leukemia. Blood 1994; 83:330-5.

20. Janssen JWG, Ludwig W-D, Borkhardt A, Spadinger U, Rieder H, Fonatsch C, et al. Pre-pre-B Acute Lymphoblastic Leukemia: High frequency of alternatively spliced ALL1-AF4 Transcripts and absence of minimal residual disease during complete remission. Blood 1994; 84:3835-42

21. Rowley JD (1992). The der(11) chromosome contains the critical breakpoint junction in the 4;11, 9;11, and 11;19 translocations in acute leukemia. Genes Chromosomes Cancer 1992; 5:264-6.

22. Marschalek R, Greil J, Lochner K, Nilson I, Siegler G, Zweckbronner, et al. Molecular analysis of the chromosomal breakpoint and fusion transcripts in the acute lymphoblastic SEM cell line with the chromosomal translocation t(4;11).Br J Haematol 1995; 90:308-20.

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