Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » By Affiliations
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top 20 Journal Cited
 » Top 20 Cited
 » Top 20 Author Cited
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Ruslan Abdullah, and Alizah Zainal, and Wee, Yew Heng, and Leaw, Chui Li, and Yeap, Chee Beng, and Lee, Mei Phing, and Salwa Abdullah Sirajuddin, and Yap, Winnie Soo Ping, and Juanita Lourdes Joseph, and Siti Azma Jusoh, and Muhammad Rashdan Muad, (2005) Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies. Electronic Journal of Biotechnology, 8 (1). pp. 25-34. ISSN 07173458

Full text not available from this repository.

Official URL: http://www.ejbiotechnology.info/content/vol8/issue1/full/1/1.pdf

Affiliations

Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology
Multimedia University Malaysia
TropBio Research Sdn Bhd
Universiti Kebangsaan Malaysia, Institute for Molecular Medicine
Universiti Kebangsaan Malaysia, Faculty of Science and Technology

Abstract

Oil palm (Elaeis guineensis Jacq.) is the highest yielding
oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic
transformation studies was evaluated. It was evident
that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation frequencies were comparable to other plant systems reported, with as high as 97.4% recorded for biolistic and 64.4% for Agrobacterium-mediated gene transfer. Like most moncots, oil palm tissues were less sensitive to kanamycin, geneticin and chloramphenicol. Instead, both hygromycin and phosphinotrycin were toxic 20
mg/l, making both suitable candidates for selecting putative transformants. IEs were also more responsive to in vitro manipulations as compared to other explants such as leaf and root tissues. Rapid in vitro response to callusing and embryogenesis or rapid and highly efficient direct germination resulted in a shorter culture period. This would minimize the production of abnormal clonal palms, which has been associated to chromosomal aberration due to prolonged time in culture. In addition, IEs also allows rapid and direct introduction of elite genes into breeding programs and in biclonal seed production.

Item Type:Journal
Additional Information:Financial support: Ministry of Science Technology and the Innovations, Malaysia
Keywords:Genetic transformation studies, immature embryos, in vitro culture, oil palm, plant regeneration
Subjects:Q Science
ID Code:1891

ABDULLAH, R.; CHARI, C.; WINNIE, Y.S.P. and YEUN, L.H. Transgenic oil palm with stably integrated CpTI gene confers resistance to bagworm larvae. In: VASIL, I.K. ed. Plant Biotechnology 2002 and Beyond: A celebration and a showcase. Kluwer Academic Publishers, 2003, p.163-165.

ABDULLAH, R.; YEUN L.H.; RASHDAN, M.M.; LEAW, C.L.; ALIZAH, Z.; YAP, S.P. and WEE, Y.H. Genetically modified oil palm for pest and disease control. In: Plant Protection in the Tropics: Tropical plant protection in the information age. (15-18th March, 1999, Kuala Lumpur, Malaysia). Malaysian Plant Protection.Society.

SIVAPRAGASAM, A.; ISMAIL, A.A.; SIDAM, A.K.; CHEAH, U.B.; CHUNG, G.F.; CHIA, T.H.; DZOLKHIFLI, O.; HO, T.H.; HUSSAN, A.K.; LEE, S.S., LIM, J.L.; LUM, K.Y.; MOHAMED, S.; NATHAN, G.; ONG, C.A.; VIJAYSEGARAN, S. and ZAINAL ABIDIN,

M.A. eds., 1999, p. 47-50.

AYRES, N.M. and PARK, D. Genetic transformation of rice. Critical Reviews in Plant Sciences, 1994, vol. 13, no. 3, p. 219-239.

CORLEY, R.H.V. Fifteen years experience with oil palm clones. A review of progress. In: Proceedings of the 1991 PORIM International Palm Oil Congress: Update and Vision. (20-25th September 1993, Kuala Lumpur, Malaysia). Palm Oil Research Institute of Malaysia.

JALANI, S.; ARIFFIN, D.; RAJANAIDU, N.; MOHD TAYEB, D.; PARANJOTHY, K.; MOHD BASRI, W.; HENSON, I.E. and CHANG, K.C. eds., 1993, p. 69-79.

CORLEY, R.H.V.; LEE, C.H.; LAW, L.H. and WONG, C.Y. Abnormal flower development in oil palm clones. Planter, 1986, vol. 62, p. 233-240.

CHOWDHURY, M.K.U.; PARVEEZ, G.K.A. and NORIHAN, M.S. Evaluation of five promoters for use in transformation of oil palm (Elaeis guineensis Jacq.). Plant Cell Reports, 1997, vol. 16, no. 5, p. 277-281.

CHU, C.C.; WANG, C.C.; SUN, C.S.; HSU, C.; YIN, K.C.; CHU, C.Y. and BI, F.Y. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Science Sinica, 1975, vol. 18. p. 659-668.

GAMBORG, O.L.; MURASHIGE, T.; THORPE, T.A. and VASIL, I.K. Plant tissue culture media. In vitro, 1969, vol. 12, p. 473-478.

GELVIN, S.B. Agrobacterium-mediated plant transformation: The biology behind the “gene-jokeying” tool. Microbiology and Molecular Biology Reviews, 2003, vol. 67, no. 1, p. 16-37.

JONES, L.H. An evaluation of the role of cytokinins in the development of abnormal infloresecences in oil palms (Elaeis guineensis Jacq.) regenerated from tissue culture. Journal of Plant Growth Regulation, 1995, vol. 14, no. 3, p.135-142.

JONES, L.H. Propagation of clonal palms by tissue culture. Oil Palm News, 1974, vol. 17, p. 1-8.

JALIGOT, E.; RIVAL, A.; BEULE, T.; DUSSERT, S. and VERDEIL, J.L. Somaclonal variation in oil palm (Elaeis guineensis Jacq.): the DNA methylation hypothesis. Plant Cell Reports, 2000, vol. 7, p. 684-690.

MURPHY, D.J. The future of new and genetically modified oil crops. In: JANICK, J. ed. Perspectives on new crops and new uses. ASHS Press, 1999, p. 216-219.

PARVEEZ, G.K.A. Production of transgenic oil palm (Elaeis guineensis Jacq.) using biolistic techniques. In: JAIN, S.M. and MINOCHA, S.C. eds. Molecular Biology of Woody Plants. Kluwer Academic Publishers, 2000, vol.2, p. 327-335.

PARVEEZ, G.K.A. and CHRISTOU, P. Biolistic-mediated DNA delivery and isolation of transgenic oil palm (Elaeis guineensis Jacq) embryogenic callus cultures. Journal of Oil Palm Research, 1998, vol. 10, p. 29-38.

PARVEEZ, G.K.A.; CHOWDHURY, M.K.U. and NORIHAN, M.S. Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) using the biolistic device. Industrial Crops and Products, 1997, vol. 6, p. 41-50.

RIVAL, A.; ABERLENC-BERTOSSI, F.; MORCILLO, F.; TREGEAR, J.; VERDEIL, J.L. and DUVAL, Y. Scaling-up in vitro clonal propagation through somatic embryogenesis: the case of oil palms: the example of oil palm (Elaeis guineensis Jacq.). Tissue Culture and Biotechnology, 1999, vol. 3, no. 2, p. 74-83.

RIVAL, A.; BERTRAND, L.; BEULÉ, T.; TROUSLOT, P. and LASHERMES, P. Suitability of RAPD analysis for the detection of somaclonal variants in oil palm (Elaeis guineensis Jacq.). Plant Breeding, 1998, vol. 117, no. 1, p.

73-76.

SAMBANTHAMURTHI, R.; SITI-HASNAH, P. and ROSLAN, M.N. Oil palm protoplasts: isolation, culture and microcallus formation. Plant Cell Tissue and Organ Culture, 1996, vol. 46, no. 1, p, 35-42.

SANFORD, J.C.; SMITH, F.D. and RUSSEL, J.A. Optimizing the biolistic process for different biological applications. Methods in Enzymology, 1993, vol. 217, p.483-509.

TEXEIRA, J.B.; SONDAHL, M.R. and KIRBY, E.G. Somatic embryogenesis from immature infloresecences of oil palm. Plant Cell Reports, 1994, vol. 13, no. 5, p. 247-250.

TEXEIRA, J.B.; SONDAHL, M.R. and KIRBY, E.G. Somatic embryogenesis from immature zygotic embryos of oil palm. Plant Cell, Tissue and Organ Culture, 1993, vol. 40, p. 105-111.

Repository Staff Only: item control page
 
 
 
 
 
   
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.