Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » By Affiliations
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top 20 Journal Cited
 » Top 20 Cited
 » Top 20 Author Cited
 » Usage Since Sept 2007


 
 
 

Login | Create Account

PCNA Expression In Epithelial Linings Of Odontogenic Cysts

Sudiono J., and Zain R.B., (2003) PCNA Expression In Epithelial Linings Of Odontogenic Cysts. Annals of Dentistry, 10 (1). pp. 1-5. ISSN 0128-7532

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=407

Affiliations

Trisakti University. Faculty of Dentistry. Dept. of Pathology Anatomy
University of Malaya. Faculty of Dentistry. Dept. of Oral Medicine, Oral Pathology and Periodontology

Abstract

Proliferating Cell Nuclear Antigen (PCNA) is one of the several markers of cellular proliferation. Epithelial proliferations play a significant role in the behaviour of odontogenic lesions. The objective of this study was to describe and compare the distribution of PCNA expression within the epithelial linings of odontogenic cysts. A total of 49 cases of odontogenic cysts consisting of 18 radicular cysts, 16 dentigerous cysts, 15 odontogenic keratocysts (OKCs) was studied. All tissues were processed routinely prior to embedding in paraffin. PCNA immunohistochemical staining was performed on 4 !-tm thick deparaffinized sections mounted on sialinized slides using the peroxidase antiperoxidase method. The distributions of PCNA expression in the cysts linings were noted and comparison was made qualitatively and quantitatively. PCNA labelling index was used for the quantitative assessment. The results showed that PCNA staining was distributed in the basal and supra basal cells for radicular cysts, dentigerous cysts, and OKCs. PCNA labelling index was highest in OKC (22.33±4.07). The high PCNA labelling index in OKC is indicative of high proliferative activity thus supporting previous reports of OKC as the most aggressive type of odontogenic cysts.

Item Type:Journal
Keywords:Immunostaining, PCNA, odontogenic cysts
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2013

1. El Murtadi A, Grehan D, Toner M, Mc Cartan BE. PCNA staining in syndrome and non syndrome odontogenic keratocysts. Oral Surg Oral Med Oral Pathol 1996; 81: 217-220.

2. Mighell A. Proliferating cell nuclear antigen. Oral Surg Oral Med Oral Pathol 1995; 30: 3-4.

3. Garcia RL, Coltrera MD, Gown AMI. Analysis of proliferative grade using anti PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis. Am J Patho11984; 134: 733-739.

4. Dawson AE, Norton JA, Weinber DS. Comparative assessment of proliferation and DNA content in breast carcinoma by image analysis and flow cytometry. Am J Patho11990; 136: 115-124.

5. Hall PA, Levison DA, Woods AL. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Patho11990; 162: 285-294.

6. Allegranza A, Girlando S, Arrigont GL. Proliferating cell nuclear antigen expression in central nervous system neoplasms. Virchows Arch (A) 1991; 419: 417-423.

7. Yu CC, Hall PA, Fletcher CDM, Camplejohns RS, Waseem NH, Lane DP, Levison DA. Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA). Histopathology 1991; 19: 29-33.

8. Ogawa I, Takata T, Miyauchi M, Ito H, Ijuhin N, Nikai H. Proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells in salivary gland tumors. Jpn J Oral Bioi 1992; 34:454-457.

9. Yu C and Filipe M. Update on proliferation associated antibodies applicable to formalin fixed paraffin-embedded tissue and their clinical applications. Histochem 1993; 25: 843-853.

10. Li TJ, Browne RM and Matthews JB. Quantification of PCNA + cells within odontogenic jaw cyst epithelium. J Oral Pathol Med 1994; 23:184-189.

11. Kramer IRH, Piridborg 11, Shear M. Histological typing of odontogenic tumours. Second edition. Springer-Verlag, Berlin, 1992; 34-42.

12. Regezi JA and Sciuba 1. Clinocal-pathologic correlation. Second edition. Saunders. Philadelphia, 1993; 322-343.

13. Waldron CA. "Odontogenic cyst and tumours". In Oral and Maxillofacial Pathology. Edited by Neville BW, Damm DD, Allen OM, Bouquot JE. 1995; 103-106.

14. Robinson L and Martinez MG. Unicystic ameloblastoma. A prognostically distinct entity. Cance~ 1977; 40: 278-285.

15. Gardner 1981. Plexiform Unicystic Ameloblastoma. A Diagnostic Problem in Dentigerous Cysts. Cancer 1981; 15: 1359-1363.

16. Labban NG and Aghabeigi B. A Comparative Stereologic and Ultrastructural Study of Blood Vessels in Odontogenic Keratocysts and Dentigerous Cysts. J Oral Pathol Med 1990; 19:442-446.

PCNA expression in epithelial linings of odontogenic cysts 5 17. Browne RM and Gough NG. Malignant Change in Odontogenic Cyst. Cancer. 1972; 29: 1199-1207.

18. Li TJ, Browne RM and Matthews JE. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 in unicystic ameloblastoma. J Histopathol 1995; 26: 219-228.

19. Tsuji T, Sasaki K, Kimura Y, Yamada K, Mori M, Shinozaki F. Measurement of proliferating cell nuclear antigen (PCNA) and its clinical application in oral cancers. J Oral Maxillofac Surg 1992; 21:369-372.

20. Pellegrini W, Facchetti F, Marocolo D, Salvi L, Capucci A, Tironi A, Rossi G. Assessment of cell proliferation in normal and pathological bone marrow biopsies: a study using double sequential immunophenotyping on paraffin sections. J

Histopathol 1995; 27: 397-405.

Repository Staff Only: item control page
 
 
 
 
 
   
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.