Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » By Affiliations
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top 20 Journal Cited
 » Top 20 Cited
 » Top 20 Author Cited
 » Usage Since Sept 2007


 
 
 

Login | Create Account

A Demonstration Of The Polymerase Chain Reaction Technique In Class Teaching

Yusof R., and Abdul Rahman P.S., and Rahim Z.H.A., (1999) A Demonstration Of The Polymerase Chain Reaction Technique In Class Teaching. Annals of Dentistry, 6 (1). pp. 1-3. ISSN 0128-7532

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=456

Affiliations

University of Malaysia. Faculty of Medicine.. Dept. of Biochemistry
University of Malaysia. Faculty of Medicine.. Dept. of Biochemistry
University of Malaya. Faculty of Dentistry. Dept. of Oral Biology

Abstract

The application of PCR technique in genetic screening was demonstrated using the genetic materials from buccal cells of the students in the class. Two factors were taken into consideration when designing the experiments. The DNA region to be amplified should not be associated with any disease state. This is to eliminate any emotional and ethical problems associated with the experiments. In this practical, the presence and absence of a 38 bp sequence in the intron of COLIA2 gene were studied. The students were also shown on how to analyse the presence of homozygous and heterozygous alleles and the genetic variations that might be observed in the different ethnic grciups of students. Another factor was the time taken to complete the experiment. Our experience showed that this experiment would take at least six hours to obtain and analyse the results. It is therefore suitable to be used in class teaching.

Item Type:Journal
Keywords:Polymerase Chain Reaction, DNA, buccal cells, class teaching
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2079

I. Mullis, KB and Faloona FA. Specific synthesis of DNA in vitro a polymerase-catalysed chain reaction. Methods Enzymol1987; 155: 335-350.

2. Erlich, HA (Editor). PCR technology: Principles and application for DNA amplification 1989; Stockton Press, New York, New York.

3. Innis, MA, Gelfand, DH, Sninsky, JJ and White TJ (Editors). PCR protocols: A guide to methods and applications 1990; Academic Press, San Diego.

4. Kurisu K & Tabata MJ. Oral Diseases 1977; 3: 223-228. Macmillan Publishers Ltd, 1977.

5. Watson CJ & Dalgleish R. PCR detection of a 38 bp length variant in the COL IA2 gene. Nucl Acids Res 1990; 18: 5925.

6. Dalgleish R, Williams G and Hawkins JR. Length polymprphism in the pro alpha 2(1) collagen gene: an altemative explanation in a case of Marfan Syndrome. Hum Genet 1986; 73: 91-92.

7. Palmer BR and Healy JP . Development of a practical illustrating the use of the polymerase chain reaction for genetic testing. Biochem Education 1993; 21 (2):106-107.

Repository Staff Only: item control page
 
 
 
 
 
   
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.