Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » By Affiliations
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top 20 Journal Cited
 » Top 20 Cited
 » Top 20 Author Cited
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Calmodulin May Regulate Proliferation of Prostate Cancer Cells

Salih, Falah A.M., and Sami, Alkatib, (2008) Calmodulin May Regulate Proliferation of Prostate Cancer Cells. Malaysian Journal of Biochemistry and Molecular Biology, 16 (1). pp. 27-29. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=601

Affiliations

International Islamic University, Kuantan. Faculty of Medicine. Dept. of Basic Medical Sciences
Kufa University, Iraq. College of Medicine. Dept. of Pathology

Abstract

In the present study, we examined the effects of calmodulin (CaM) antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) on androgen-sensitive, LNCaP, and androgen-independent, PC-3, prostate cancer cell lines. Proliferation of LNCaP cells was six times more sensitive to the inhibitory effect of W-7 than were PC-3 cells. To explore the molecular mechanism for this inhibition, the relative amounts of CaM in LNCaP and PC-3 cells were analysed. Western blot analysis revealed two and three fold greater amounts of CaM in the nuclear and cytoplasmic fractions, respectively, of LNCaP cells than in those of PC-3 cells. These studies provide indirect evidence for the biological interaction between androgen receptor (AR) and CaM and suggest the potential usefulness of CaM antagonists in blocking AR activity in prostate cancer. It also suggested the possible involvement of CaM in regulating proliferation of prostate cancer cells. This is a preliminary report and further understanding of the molecular mechanism of Ca2+/ CaM action in prostate cancer is required.

Item Type:Journal
Keywords:androgen receptor, calmodulin, gene regulation, prostate cancer
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2171

1. Li L, Sacks DB. Functional interactions between calmodulin and estrogen receptor-alpha. Cell Signal 2007; 19(3): 439-443.

2. Krishnaraju K, Murugesan K, Vij U, Kapur BM, Farooq A. Calmodulin levels in oestrogen receptor positive and negative human breast tumors. Br J Cancer 1991; 63: 346-347.

3. Li L, Li Z, Sacks DB. Calmodulin regulates the transcriptional activity of estrogen receptors. Selective inhibition of calmodulin functions in subcellular compartments. J Biol Chem 2003; 278(2): 1195-1200.

4. Falah AMS, Alabadi R. Possible role of calmodulin in gene activation and regulation. Int Med J 2004; 3: 2.

5. Huggins C. Endocrine-induced regression of cancers. Science 1967; 156: 1050-1054.

6. Reddy GPV. Catalytic function of thymidylate synthase is confined to S phase due to its association with replitase. Biochem Biophys Res Commun 1982 15; 109(3): 908-915.

7. Subramanyam C, Honn SC, Reed WC, Reddy GP. Nuclear localization of 68 kDa calmodulin-binding protein is associated with the onset of DNA replication. J Cell Physiol 1990; 144(3):423-8.

8. Sacks DB, Porter SE, Ladenson JH, McDonald JM. Monoclonal antibody to calmodulin. Development, characterization, and comparison with polyclonal anticalmodulin antibodies. Anal Biochem 1991; 194: 369-377.

9. Zhou WRT, Riffat H, Xiaoyan C. Genes regulated by androgen in the rat ventral prostate. Proc. Natl. Acad. Sci. USA 1997; 94: 12999-13004.

Repository Staff Only: item control page
 
 
 
 
 
   
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.