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The Use of Denaturing HPLC as a Rapid Screening Method for Detection of Polymorphisms in Exon 26 of the Multi Drug Resistance Gene, MDR1, in Malay Patients with Leukemia

Badrul Hisham Y., and Rosline H., and Abdul Aziz B., and Narazah M.Y., (2008) The Use of Denaturing HPLC as a Rapid Screening Method for Detection of Polymorphisms in Exon 26 of the Multi Drug Resistance Gene, MDR1, in Malay Patients with Leukemia. Malaysian Journal of Biochemistry and Molecular Biology, 16 (1). pp. 30-33. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=602

Affiliations

Universiti Sains Malaysia. School of Medical Sciences. Human Genome Center
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Haematology
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Medicine
Universiti Sains Malaysia. Advanced Medical and Dental Institute

Abstract

We have screened for 3435C>T polymorphism in exon 26 of the highly expressed, multi drug resistance gene, MDR1, in 101 Malay patients with leukemia using denaturing high performance liquid chromatography (dHPLC). The frequencies for the homozygous wild type, heterozygous mutation and homozygous mutation were 33.7%, 50.5% and 15.8%, respectively. The difference between the types of leukemia and sex of patients for the distribution of 3435C>T polymorphism was statistically not significant. The technique was able to confirm the presence of known 3435C>T polymorphism in exon 26 in all patients.

Item Type:Journal
Keywords:MDR1 gene, denaturing HPLC, Malays, leukemia, polymorphism
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2172

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