Author, Subjects, Keywords

Cited Author

 

 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » By Affiliations
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top 20 Journal Cited
 » Top 20 Cited
 » Top 20 Author Cited
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Overview of Proteomics

Onn Haji Hashim, (2007) Overview of Proteomics. Malaysian Journal of Biochemistry and Molecular Biology, 15 (1). pp. 1-7. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=575

Affiliations

University of Malaya. Faculty of Medicine. Dept. of Molecular Medicine

Abstract

The era of genomics has brought tremendous advancement especially in the fields of medicine and life sciences. Despite the overwhelming growth of information generated from genomics research, a huge gap specifically in relation to genome expression persists, and became increasingly noticeable. This has sparked interest in studies of proteins expression and eventually added limelight to the field of proteomics. Aside from the need to fill the gap, the emergence of proteomics is also deemed to have occurred due to the advancement in capabilities of research techniques, particularly in the separation and identification of proteins. Proteomics has since progressed and is slowly extending into other research as well as applied subspecialties. This brief overview was written to provide a basic and simplified understanding of proteomics in view of its growing interest from newcomers to the area.

Item Type:Journal
Keywords:proteomics
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2179

1. Wilkins MR, Sanchez JC, Cooley AA, Appel RD, Humphery-Smith I, Hochstrasser DF, Williams KL. Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Genet Eng Rev 1995; 13: 19-50.

2. Wasinger VC, Cordwell SJ, Cerpa-Poljak A, Yan JX, Cooley AA, Wilkins MR, Duncan MW, Harris R, Williams KL, Humphery-Smith I. Progress with gene product mapping of the Mollicutes: Mycoplasma genitalium. Electrophoresis 1995; 16: 1090-1094.

3. Raffin-Sanson ML, de Keyzer Y, Bertagna X. Proopiomelanocortin, a polypeptide precursor with multiple functions: from physiology to pathological conditions. Eur J Endocrinol 2003; 149: 79-90.

4. Anderson L, Seilhamer J. A comparison of selected mRNA and protein abundances in human liver. Electrophoresis 1997; 18: 533-537.

5. Gygi SP, Rochon Y, Franza BR, Aebersold R. Correlation between protein and mRNA abundance in yeast. Mol Cell Biol 1999; 19: 1720-1730.

6. Ideker T, Thorsson V, Ranish JA, Christmas R, Buhler J, Eng JK, Bumgarner R, Goodlett DR, Aebersold R, Hood L. Integrated genomic and proteomic analyses of systematically perturbed metabolic network. Science 2001;292: 929-934.

7. O’Farell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975; 250: 4007-4021.

8. Scheele GA. Two-dimensional gel analysis of soluble proteins. Characterization of guinea pig exocrine pancreatic proteins. J Biol Chem 1975; 250: 5375-5385.

9. Anderson NG, Anderson NL. Twenty years of twodimensional electrophoresis: past, present and future. Electrophoresis 1996; 17: 443-453.

10. Westermeier R, Naven T. Expression Proteomics In: Proteomics in practice, a laboratory manual of proteome analysis. New York: Wiley 2002; 11-57.

11. Bjellqvist B, Pasquali C, Ravier F, Sanchez JC, Hochstrasser D. A non linear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale. Electrophoresis 1993; 14: 1357-1365.

12. Doustjalali SR, Yusof R, Yip CH, Looi LM, Pillay B, Hashim OH. Aberrant expression of acute-phase reactant proteins in sera and breast lesions from patients with malignant and benign breast tumors. Electrophoresis 2004; 25: 2392-2401.

13. Doustjalali SR, Yusof R, Govindasamy GK, Bustam AZ, Pillay B, Hashim OH. Patients with nasopharyngeal carcinoma demonstrate enhanced serum and tissue ceruloplasmin expression. J Med Invest 2006; 53: 20-28.

14. Blackstock WP, Weir MP. Proteomics: quantitative and physical mapping of cellular proteins. Trends Biotechnol 1999; 17: 121-127.

15. Rout MP, Aitchison JD, Suprapto A, Hjertaas K, Zhao Y, Chait BT. The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 2000; 148: 635-651.

16. LaBaer J, Ramachandran N. Protein microarrays as tools for functional proteomics. Curr Opin Chem Biol 2005; 9: 14-19.

17. Miller JC, Zhou H, Kwekel J, Cavallo R, Burke J, Butler EB, Teh BS, Haab BB. Antibody microarray profiling of human prostate cancer sera: antibody screening and identification of potential biomarkers. Proteomics 2003;3: 56-63.

18. Lewis TS, Hunt JB, Aveline ID, Jonscher KR, Louie DF, Yeh JM, Nahreini TS, Resing KA, Ahn NG. Identification of novel map kinase pathway signaling targets by functional proteomics and mass spectrometry. Mol Cell 2000; 6: 1343-1354.

19. Wu J, Lenchik NJ, Pabst MJ, Solomon SS, Shull J, Gerling IC. Functional characterization of two-dimensional gelseparated proteins using sequential staining. Electrophoresis 2005; 26, 225-237.

20. Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W. The current state of twodimensional electrophoresis with immobilized pH gradients. Electrophoresis 2000; 21: 1037-1053.

21. Celis JE, Gromov P. 2D protein electrophoresis: can it be perfected? Curr Opin Biotechnol 1999; 10: 16-21.

22. Anderson NL, Anderson NG. The human plasma proteome: history, character and diagnostic prospects. Mol Cell Proteomics 2002; 1: 845-867.

23. Link AJ, Eng J, Schieltz DM, Carmack EB, Mize GJ,Morris R, Garvik BM, Yates JR. Direct analysis of protein complexes using mass spectrometry. Nat Biotecnol 1999;17: 676-682.

24. Figeys D, Corthals GL, Gallis B, Goodlett DR, Ducret A, Corson MA, Aebersold R. Data-dependent modulation of solid-phase extraction capillary electrophoresis for the analysis of complex peptide and phosphopeptide mixtures by tandem mass spectrometry: application to endothelial nitric oxide synthase. Anal Chem 1999; 71: 2279-2287.

25. Opitech GJ, Lewis KC, Jorgenson JW, Anderregg RJ. Comprehensive on-line LC/LC/MS of proteins. Anal Chem 1997; 69: 1518-1524.

26. Rosenfeld J, Capdevielle J, Guillemot JC, Ferrara P. In-gel digestion for proteins for internal sequence analysis after one- or two-dimensional electrophoresis. Anal Biochem

1992; 203: 173-179.

27. Courchesne PL, Luethy R, Patterson SD. Comparison of in-gel and on-membrane digestion methods at low to subpmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorbtion/ionization mass spectrometry. Electrophoresis 1997; 18: 369-381.

28. MacDonald JA, Borman MA, Muranyi A, Somlyo AV, Hartshorne D, Haystead TA. Identification of the endogenous smooth muscle myosin phosphatase-associated kinase. Proc Natl Acad Sci USA 2001; 98: 2419-2424.

29. Yates JR. Mass spectrometry and the age of the proteome. J Mass Spectrom 1998; 33: 1-19.

30. Karas M, Hillenkamp F. Laser desorbtion ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 1988; 60: 2299-2301.

31. Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM. Electrospray ionization for mass spectrometry of large biomolecules. Science 1989; 246: 64-71.

32. Merchant M, Weinberger SR. Recent advancements in surface-enhanced laser desorbtion/ionization-time of flightmass spectrometry. Electrophoresis 2000; 21: 1164-1167.

33. Yates JR. Protein structure analysis by mass spectrometry.Methods Enzymol 1996; 271: 351-377.

34. Shevchenko A, Chernushevich I, Ens W, Standing KG, Thomson B, Wilm M, Mann M. Rapid de novo peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer. Rapid Commun Mass Spectrom 1997; 11: 1015-1024.

35. Jonscher KR, Yates JR. The quadrupole ion-trap mass spectrometer - a small solution to a big challenge. Anal Biochem 1997; 72: 4266-4274.

36. Martin SE, Shabanowitz J, Hunt DF, Marto JA. Subfemtomole MS and MS/MS peptide sequence analysis using nano-HPLCESI Fourier-transform ion cyclotron resonance mass spectrometry. Anal Chem 2000; 72: 4266-4274.

37. Swatton JE, Prabakaran S, Karp NA, Lilley KS, Bahn S. Protein profiling of human postmortem brain using 2-

dimensional fluorescence difference gel electrophoresis (2-

D DIGE). Mol Psychiatry 2004; 9, 128-143.

38. Jiang H, English, AM. Quantitative analysis of the yeast proteome by incorporation of isotopically labeled Leucine. J Proteome Res 2002; 1: 345-350.

Repository Staff Only: item control page