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Influences of Hepatitis B Virus (HBV) Genotypes, Core Promoter Mutations and HBeAg/ anti-HBe Titers on HBV DNA Levels

Lim. Chee Kent, and Low, Hsin Mei, and Tan, Joanne Tsui Ming, and Khoo, Jason Boo Siang, and Ravichandran, Aarthi, (2007) Influences of Hepatitis B Virus (HBV) Genotypes, Core Promoter Mutations and HBeAg/ anti-HBe Titers on HBV DNA Levels. Malaysian Journal of Biochemistry and Molecular Biology, 15 (2). pp. 63-71. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=570

Affiliations

Monash University Malaysia. School of Arts and Sciences
Monash University, Clayton, Australia. Faculty of Medicine, Nursing and Health Sciences
University of Sydney. Discipline of Medicine

Abstract

The influences of hepatitis B virus (HBV) genotypes and core promoter mutations (A1762G1764→T1762A1764) on HBV DNA levels in HBV-infected serum were investigated. The correlations between the titers of HBV e antigen (HBeAg) and its corresponding antibody with HBV DNA levels were also studied. Seventy HBV-infected serum samples were utilized for the study. Genotyping, core promoter mutations and precore stop codon mutations were analyzed using polymerase chain reaction (PCR) and restriction fragments length polymorphism (RFLP). Serological markers were quantified using enzyme-linked immunosorbent assays. HBV DNA levels were determined with real-time PCR. Our data did not yield significant correlation between HBV DNA with the genotypes B and C (median: 1.68 x 107 vs. 2.98 x 108 copies/mL; P=0.69) as well as with the core promoter mutations (median: 3.85 x 105 vs. 7.02 x 105 copies/mL; P=0.37). No precore stop codon mutant was detected in our study. HBeAg titers were positively correlated to HBV DNA levels (R2=0.2549; P=0.001) but this was not observed for its corresponding antibody (R2=0.0034; P=0.24). Our data suggest that viral genotypes and the core promoter mutations may not have a direct influence on HBV DNA levels.

Item Type:Journal
Keywords:HBV DNA levels, genotypes, core promoter mutations, HBeAg, anti-HBe
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2183

1. Lok AS. Chronic hepatitis B. N Engl J Med 2002; 346:1682-1683.

2. Sablon E, Shapiro F. Advances in molecular diagnosis of HBV infection and drug resistance Int J Med Sci 2005; 2:8-16.

3. Whalley SA, Murray JM, Brown D, Webster GJ, Emery VC, Dusheiko GM, Perelson AS. Kinetics of acute hepatitis B virus infection in humans. J Exp Med 2001; 193: 847-854.

4. Lok ASF, McMahon BJ. Chronic hepatitis B. Hepatology 2001; 34: 1225-1241.

5. Yang HI, Lu SN, Liaw YF, You SL, Sun CA, Wang LY, Hsiao CK, Chen PJ, Chen DS, Chen CJ, Taiwan Community-Based Cancer Screening Project Group. Hepatitis B e antigen and the risk of hepatocellular carcinoma. N Engl J Med 2002; 347: 168-174.

6. Yu MW, Yeh SH, Chen PJ, Liaw YF, Lin CL, Liu CJ, Shih WL, Kao JH, Chen DS, Chen CJ. Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men. J Natl Cancer I 2005; 97: 265-272.

7. Chen CJ, Yang HI, Su J, Jen CL, You SL, Lu SN, Huang GT, Iloeje UH. Risk of hepatocellular carcinoma across a biological gradient of serum hepatitis B virus DNA level. JAMA 2006; 295: 65-73.

8. Chan HLY, Tsang SWC, Liew CT, Tse CH, Wong ML, Ching JYL, Leung NWY, Tam JSL, Sung JJY. Viral genotype and hepatitis B virus DNA levels are correlated with histological liver damage in HBeAg-negative chronic hepatitis B virus infection. Am J Gastroenterol 2002; 97:406-412.

9. Lindh M, Horal P, Dhillon AP, Norkrans G. Hepatitis B virus DNA levels, precore mutations, genotypes and histological activity in chronic hepatitis B. J Viral Hepat 2000; 7: 258-267.

10. Tong MJ, Blatt LM, Kao JH, Cheng JT, Corey WG. Precore/ basal core promoter mutants and hepatitis B viral DNA levels as predictors for liver deaths and hepatocellular carcinoma. World J Gastroenterol 2006; 12: 6620-6626.

11. Yuen MF, Ng IO, Fan ST, Yuan HJ, Wong DK, Yuen JC, Sum SS, Chan AO, Lai CL. Significance of HBV DNA levels in liver histology of HBeAg and Anti-HBe positive patients with chronic hepatitis B. Am J Gastroenterol 2004;99: 2032-2037.

12. Yuan HJ, Yuen MF, Wong DKH, Sablon E, Lai CL. The relationship between HBV-DNA levels and cirrhosis-related complications in Chinese with chronic hepatitis B. J Viral Hepat 2005; 12: 373-379.

13. Mendy ME, Kaye S, van der Sande M, Rayco-Solon P, Waight PA, Shipton D, Awi D, Snell P, Whittle H, McConkey SJ. Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in the Gambia. Virol J 2006; 3: 23.

14. Krogsgaard K, Kryger P, Aldershvile J, Andersson P, Brechot C. Hepatitis B virus DNA in serum from patients with acute hepatitis B. Hepatology 1985; 5: 10-13.

15. Jardi R, Buti M, Rodriguez-Frias F, Cortina M, Esteban R, Guardia J, Pascual C. The value of quantitative detection

of HBV-DNA amplified by PCR in the study of hepatitis B infection. J Hepatol 1996; 24: 680-685.

16. Lindh M, Hannoun C, Dhillon AP, Norkrans G, Horal P. Core promoter mutations and genotypes in relation to viral replication and liver damage in East Asian hepatitis B virus carriers. J Infect Dis 1999; 179: 775-782.

17. Kao JH, Chen PJ, Lai MY, Chen DS. Clinical and virological aspects of blood donors infected with hepatitis B virus genotypes B and C. J Clin Microbiol 2002; 40: 22-25.

18. Peng XM, Huang GM, Li JG, Huang YS, Mei YY, Gao ZL. High level of hepatitis B virus DNA after HBeAg-toanti-HBe seroconversion is related to coexistence of mutations in its precore and basal core promoter. World J Gastroenterol 2005; 11: 3131-3134.

19. Pang A, Yuen MF, Yuan HJ, Lai CL, Kwong YL. Realtime quantification of hepatitis B virus core-promoter and pre-core mutants during hepatitis E antigen seroconversion. J Hepatol 2004; 40: 1008-1017.

20. Carman W, Jacyna M, Hadziyannis S, Karayiannis P, McGarvey M, Makris A, Thomas H. Mutation preventing formation of the hepatitis B e antigen in patients with chronic hepatitis B infection. Lancet 1989; 2: 588-591.

21. Okamoto H, Tsuda F, Akahane Y, Sugai Y, Yoshiba M, Moriyama K, Tanaka T, Miyakawa Y, Mayumi M. Hepatitis B virus with mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen. J Virol 1994; 68: 8102-8110.

22. Brunetto MR, Giarin MM, Oliveri F, Chiaberge E, Baldi M, Alfarano A, Serra A, Saracco G, Verme G, Will H, Bonino F. Wild-type and e antigen-minus hepatitis B viruses and course of chronic hepatitis. Proc Natl Acad Sci USA 1991; 88: 4186-4190.

23. Lim CK, Tan JTM, Khoo JBS, Ravichandran A, Low HM, Chan YC, Ton SH. Correlations of HBV genotypes, mutations affecting HBeAg expression and HBeAg/ anti-HBe status in HBV carriers. Int J Med Sci 2006; 3: 14-20.

24. Lindh M, Andersson AS, Gusdal A. Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus—largescale analysis using a new genotyping method. J Infect Dis 1997; 175: 1285-1293.

25. Lindh M, Gonzalez JE, Norkrans G, Horal P. Genotyping of hepatitis B virus by restriction pattern analysis of a re-S amplicon. J Virol Methods 1998; 72: 163-174.

26. Naito H, Hayashi S, Abe K. Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers. J Clin

Microbiol 2001; 39: 362-364.

27. Yuh CH, Chang YL, Ting LP. Transcriptional regulation of

precore and pregenomic RNAs of hepatitis B virus. J Virol

1992; 66: 4073-4084.

28. Takahashi K, Aoyama K, Ohno N, Iwata K, Akahane Y,

Baba K, Yoshizawa H, Mishiro S. The precore/core

promoter mutant (T1762A1764) of hepatitis B virus: clinical

significance and an easy method for detection. J Gen Virol

1995; 76: 3159-3164.

29. Lindh M, Furuta Y, Ljunggren KK, Norkrans G, Horal P.

Detection of hepatitis B virus precore TAG mutant by an

amplification-created restriction site method. J Infect Dis 1995; 171: 194-197.

30. Lole KS, Arankalle VA. Quantitation of hepatitis B virus DNA by real-time PCR using internal amplification control and dual TaqMan MGB probes. J Virol Methods 2006; 135: 83-90.

31. Chu CJ, Hussain M, Lok ASF. Quantitative serum HBV DNA levels during different stages of chronic hepatitis B infection. Hepatology 2002; 36: 1408 -1415.

32. Chen CH, Lee CM, Lu SN, Changchien CS, Eng HL, Huang CM, Wang JH, Hung CH, Hu TH. Clinical significance of hepatitis B virus (HBV) genotypes and precore and core promoter mutations affecting HBV e antigen expression in Taiwan. J Clin Microbiol 2005; 12:6000-6006.

33. Ho SK, Yam WC, Leung ET, Wong LP, Leung JK, Lai KN, Chan TM. Rapid quantification of hepatitis B virus DNA by real-time PCR using fluorescent hybridization probes. J Med Microbiol 2003; 52: 397-402.

34. Toan NL, Song le H, Kremsner PG, Duy DN, Binh VQ, Koeberlein B, Kaiser S, Kandolf R, Torresi J, Bock CT. Impact of the hepatitis B virus genotype and genotype mixtures on the course of liver disease in Vietnam. Hepatology 2006; 43: 1375-1384.

35. Brunetto MR, Oliveri F, Coco B, Leandro G, Colombatto P, Gorin JM, Bonino F. Outcome of anti-HBe positive chronic hepatitis B in alpha-interferon treated and untreated patients: a long term cohort study. J Hepatol 2002; 36: 263-270.

36. Parekh S, Zoulim F, Ahn SH, Tsai A, Li J, Kawai S, Khan N, Trepo C, Wands J, Tong S. Genome replication, virion secretion, and e antigen expression of naturally occurring hepatitis B virus core promoter mutants. J Virol 2003; 77:6601-6612.

37. Whittle H, Inskip H, Bradley AK, McLaughlan K, Shenton F, Lamb W, Eccles J, Baker BA, Hall AJ. The pattern of childhood hepatitis B infection in two Gambian villages. J Infect Dis 1990; 161: 1112-1115.

38. Huang YH, Wu JC, Chang TT, Sheen IJ, Huo TI, Lee PC, Su CW, Lee SD. Association of core promoter/precore mutations and viral load in e antigen-negative chronic hepatitis B patients. J Viral Hepat 2006; 13: 336-342.

39. Orito E, Mizokami M, Sakugawa H, Michitaka K, Ishikawa K, Ichida T, Okanoue T, Yotsuyanagi H, Iino S. A casecontrol study for clinical molecular biological differences between hepatitis B virus of genotypes B and C. Hepatology 2001; 33: 218-223.

40. Liu CJ, Chen PJ, Lai MY, Lin FY, Wang T, Kao JH, Chen DS. Viral factors correlate with hepatitis B e antigen seroconverson in patients with chronic hepatitis B. Liver Int 2006; 26: 949-955.

41. Sugauchi F, Orito E, Ichida T, Kato H, Sakugawa H, Kakumu S, Ishida T, Chutaputti A, Lai CL, Ueda R, Miyakawa Y, Mizokami M. Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene. J Virol 2002; 76: 5985-5992.

42. Sugauchi F, Orito E, Ichida T, Kato H, Sakugawa H, Kakumu S, Ishida T, Chutaputti A, Lai CL, Gish RG, Ueda R, Miyakawa Y, Mizokami M. Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C. Gastroenterology 2003; 124: 925-932.

43. Bowyer SM, van Staden L, Kew MC, Sim JG. A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa. J Gen Virol 1997; 78: 1719-1729.

44. Kramvis A, Kew M, Francois G. Hepatitis B virus genotypes. Vaccine 2005; 23: 2409-2423.

45. Norder H, Courouce AM, Coursaget P, Echevarria JM, Lee SD, Mushahwar IK, Robertson BH, Locarnini S, Magnius LO. Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes. Intervirology 2004; 47: 289-309.

46. Sugiyama M, Tanaka Y, Kato T, Orito E, Ito K, Acharya SK, Gish RG, Kramvis A, Shimada T, Izumi N, Kaito M, Miyakawa Y, Mizokami M. Influence of hepatitis B virus genotypes on the intra and extracellular expression of viral DNA and antigens. Hepatology 2006; 44: 915-924.

47. Gandhe SS, Chadha MS, Walimbe AM, Arankalle VA. Hepatitis B virus: prevalence of precore/core promoter mutants in different clinical categories of Indian patients. J Viral Hepat 2003; 10: 367-382.

48. Baumert TF, Rogers SA, Hasegawa K, Liang TJ. Two core promoter mutations identified in a hepatitis B virus strain associated with fulminant hepatitis result in enhanced viral replication. J Clin Invest 1996; 98: 2268-2276.

49. Chun YK, Kim JY, Woo HJ, Oh SM, Kang I, Ha J, Kim SS. No significant correlation exists between core promoter mutations, viral replication, and liver damage in chronic hepatitis B infection Hepatology 2000; 32: 1154-1162.

50. Lin CL, Liao LY, Liu CJ, Chen PJ, Lai MY, Kao JH, Chen DS. Hepatitis B genotypes and precore/basal core promoter mutants in HBeAg-negative chronic hepatitis B. J Gastroenterol 2002; 37: 283-287.

51. Yamaura T, Tanaka E, Matsumoto A, Rokuhara A, Orii K, Yoshizawa K, Miyakawa Y, Kiyosawa K. A case control study for early prediction of hepatitis B e antigen seroconversion by hepatitis B virus DNA levels and mutations in the precore region and core promoter. J Med Virol 2003; 70: 545-552.

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