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Isolation of Perchloric-acid Soluble Protein from Normal Human Monocytes

Mat Ludin Che Mat, and Hasenan Nordin, and Wan Maziah Wan Mohamed, (2007) Isolation of Perchloric-acid Soluble Protein from Normal Human Monocytes. Malaysian Journal of Biochemistry and Molecular Biology, 15 (2). pp. 80-83. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=573

Affiliations

Universiti Sains Malaysia. School of Medical Sciences. Academic Laboratory Unit
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Chemical Pathology
Universiti Sains Malaysia. School of Medical Sciences. Dept.of Pediatrics

Abstract

Perchloric-acid-soluble protein (PSP), with molecular mass of approximately 14.5 kDa, was isolated from peripheral blood monocytes of healthy donors by a combination of perchloric acid solubilization, trichloroacetic acid and ammonium sulfate extraction, and CM-Sephadex chromatography techniques. However, this protein was not found in all cultured blast cells of acute myeloblastic leukemia patients that were tested. The isolated protein showed strong cross-reactivity when tested with PSP antibody, suggesting close similarity to the p14.5 PSP found in human mononuclear phagocytes isolated by earlier workers. PSP did not exert any significant effect on the colony forming and proliferative activities of cultured blast cells from patients with acute myeloblastic leukemia.

Item Type:Journal
Additional Information:We are very grateful to Associate Professor Mustapha Musa, Head of Immunology Department for use of laboratory and equipment. We are indebted to Ms Malisa Yoong Abdullah, Ms Kamariah Abdul Rahim and Mr Abdul Razak, from the Immunology laboratory, and Ms Asiah from the cell culture section, PPSG, for their technical assistance during the entire course of this study. The research was financially supported by a short-term grant (304/PPSP/6131243) from Universiti Sains Malaysia.
Keywords:Acute leukemia; perchloric-acid-soluble protein; blast cells; monocytes
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2186

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