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Chimerism Analysis of a Patient with Acute Lymphoblastic Leukemia after Allogeneic Haematopoeitic Stem Cell Transplantation: A Preliminary Report

Badrul Hisham Y., and Aliza M.Y., and Zaiton Y., and Puteri J.N., and Norsiah M.D., and Hishamshah I., and Zubaidah Z., (2007) Chimerism Analysis of a Patient with Acute Lymphoblastic Leukemia after Allogeneic Haematopoeitic Stem Cell Transplantation: A Preliminary Report. Malaysian Journal of Biochemistry and Molecular Biology, 15 (2). pp. 84-87. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=574

Affiliations

Institute for Medical Research Malaysia. Cancer Research Centre
Institute for Medical Research Malaysia. Cancer Research Centre
Institute for Medical Research Malaysia. Cancer Research Centre
Institute for Medical Research Malaysia. Cancer Research Centre
Specialised Diagnostic Centre. Specialised Diagnostic Centre
Hospital Kuala Lumpur. Institute of Pediatrics
Institute for Medical Research Malaysia. Cancer Research Centre

Abstract

A chimerism analysis for monitoring donor DNA engraftment in allogeneic peripheral blood stem cell transplantation (PBSCT) in a patient with acute lymphoblastic leukaemia (ALL) at day 14 (D14) and day 28 (D28) of post-PBSCT was performed. Five specific sequence polymorphisms located in five different chromosomes were selected for this study. Only one marker (S02) was able to discriminate DNA profiles between the donor and the recipient. This particular marker was negative in the recipient but positive in the donor. By using 15 serial-halved dilutions of mixed DNAs using TaqMan technology, a linear correlation (r2) of 0.97 was obtained. In the post-PBSCT analysis, we managed to detect presence of the donor DNA profile at D14, which was slightly reduced at D28. The Ct values were 27.01 at D14 and 29.38 at D28. The percentage of donor DNA detected in the recipient’s DNA decreased from 37.18% at D14 to 30.68% at D28.

Item Type:Journal
Keywords:Chimerism, Real-Time PCR, peripheral blood stem cell transplantation (PBSCT), acute lymphoblastic leukemia
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2187

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