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Molecular Characterisation of the etb70 Gene Encoding a Putative Profilin in Eimeria tenella

Soon, Puay-Eng, and Mohd. Sanusi Jangi, and Wan, Kiew-Lian, (2006) Molecular Characterisation of the etb70 Gene Encoding a Putative Profilin in Eimeria tenella. Malaysian Journal of Biochemistry and Molecular Biology, 14 (1). pp. 14-16. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=580

Affiliations

Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Biosciences and Biotechnology
Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Biosciences and Biotechnology
Universiti Kebangsaan Malaysia. Faculty of Science and Technology. School of Biosciences and Biotechnology

Abstract

Actin-binding proteins, such as profilins, are believed to play an important role in the gliding motility and subsequently host cell invasion in apicomplexan parasites. In this study, we report the characterisation of an Eimeria tenella gene, etb70, which was initially identified as an expressed sequence tag locus. Analysis of the complete cDNA sequence of etb70 shows that the most possible open reading frame consists of 507 bp and codes for a 169-amino acid residue protein with a predicted molecular size of ~18.4 kDa. Comparison of the etb70 sequence to other sequences in the database reveals significant matches with profilins from a variety of organisms, while sequence alignment analysis showed that etb70 is highly similar to other profilin molecules. The etb70 gene was expressed as a recombinant protein, and further analysis demonstrated that recombinant etb70 is capable of binding to actin. Thus, taking into consideration the experimental data obtained, the results indicate that etb70 is a profilin homologue in E. tenella.

Item Type:Journal
Keywords:actin-binding, profilin, expressed sequence tag, recombinant protein, coccidiosis
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2188

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