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Transfection of HT-29 and Hep-2 with IL-4R cDNA Increases the Sensitivity of the Cells Towards IL-4

Adawiyah Suriza S., and Mustafa M,, and Ishak M., (2006) Transfection of HT-29 and Hep-2 with IL-4R cDNA Increases the Sensitivity of the Cells Towards IL-4. Malaysian Journal of Biochemistry and Molecular Biology, 14 (1). pp. 13-17. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=582

Affiliations

University of Malaya. Faculty of Science. 1Institute of Biological Sciences
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Immunology
Universiti Sains Malaysia. School of Pharmaceutical Sciences

Abstract

Transfection of HT-29 and Hep-2 cells with cDNA of the IL-4 receptor (IL-4R) was demonstrated to result in increased number of functional IL-4R being expressed on the surface of the cells. When exposed to IL-4, marked differences in the percentage of relative cell viability were detected in the transfected HT-29 (HT-29t) and Hep-2 (Hep-2t) cells as compared to their untransfected counterparts. While a significant decreased in the percentage of relative cell proliferation was observed in the HT-29t cells, the percentage of relative cell proliferation was significantly increased in the Hep-2t cells. This confirmed the antagonistic effects of IL-4 on different types of cells. In general, our data also demonstrated that the increase of IL-4R expression rendered HT-29 and HEP-2 cells to be more sensitive to IL-4 and that lower concentrations of IL-4 were required to exert its effect on the proliferation of the cells.

Item Type:Journal
Keywords:IL-4, IL-4 receptor, transfection, proliferation, carcinoma
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2190

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