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Screening for 3435C>T and 2677G>T/A Polymorphisms of Multi-Drug Resistance (MDR1) Gene in Malay Patients with Leukemia

Badrul Hisham Y., and Rosline H,, and Mohd Ros S., and Norsa’adah B., and Abdul Aziz B., and Narazah M.Y., (2006) Screening for 3435C>T and 2677G>T/A Polymorphisms of Multi-Drug Resistance (MDR1) Gene in Malay Patients with Leukemia. Malaysian Journal of Biochemistry and Molecular Biology, 14 (1). pp. 18-24. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=583

Affiliations

Universiti Sains Malaysia. School of Medical Sciences. Human Genome Centre
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Haematology
Universiti Sains Malaysia. School of Medical Sciences. Human Genome Centre
Universiti Sains Malaysia. School of Medical Sciences. Unit of Biostatistics & Research Methodology
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Medicine
Universiti Sains Malaysia. Advance Medical and Dental Institute

Abstract

The prevalence of 2677G>T and 3435C>T polymorphisms of the multi-drug resistance (MDR1) gene was found to be different in many populations and it was significantly influenced by ethnicities. The mechanism on how these two single nucleotide polymorphisms (SNPs) play a role in regulating the MDR1 expression especially in leukemia patients is still unclear and controversial. The objective of this study was to evaluate the distribution of 3435C>T and 2677G>T/A polymorphisms among Malay patients diagnosed to have leukemia (n=101) by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based assays. The genotype frequencies for homozygous genotype GG, heterozygous genotype GT and genotype GA and homozygous mutation genotype TT or AA in exon 21 were reported as 36.9%, 48.5%, 5.8% and 8.7%, respectively. In exon 26, the frequencies were 34.0%, 50.5% and 15.5% for the homozygous wild type CC, heterozygous mutation CT and homozygous mutation TT, respectively. There were no association found between the distribution of SNPs in both exons with the types of leukemia and sex of patients. Therefore, further clinical studies in a larger sample size should be carried out in order to find the association between sex and types of leukemia in distribution of common SNPs in Malay patients with leukemia. The significant implication of these common SNPs to the level of P-gp expression in Malay patients with leukemia that might contribute to the chemotherapy resistance should be carried out as a future study.

Item Type:Journal
Keywords:P-glycoprotein, Multi-drug resistance, SNPs, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Malays
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2191

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