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Comparison Between Locally Produced Chlorella vulgaris and Chlorella vulgaris from Japan on Proliferation and Apoptosis of Liver Cancer Cell Line, HepG2

Suhana Md. Saad, and Yasmin Anum Mohd Yusof, and Wan Ngah W.Z., (2006) Comparison Between Locally Produced Chlorella vulgaris and Chlorella vulgaris from Japan on Proliferation and Apoptosis of Liver Cancer Cell Line, HepG2. Malaysian Journal of Biochemistry and Molecular Biology, 13 (1). pp. 32-36. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=591

Affiliations

Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry
Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry
Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry

Abstract

Chlorella vulgaris (CV), a unicellular green algae, has been shown to have many positive effects in human and animal studies in Japan and in the United States. In this study, we cultured CV in the laboratory to study its anti-carcinogenic properties in liver cancer cell line, HepG2. At the same time we compared the anti-carcinogenic properties of locally produced CV with that from Japan. Liver cancer cell line, HepG2 and normal liver cell line, WRL68, were treated with hot water extract of CV cultured in the laboratory (CVL) and with hot water extract of CV from Japan (CVJ). Results from the BrdU assay showed that CVJ increased proliferation of HepG2 cells initially and reduced later at higher concentration of CVJ extract. However, HepG2 cell line treated with hot water extract of CVL showed significant decrease in proliferation rate ; with a 50% reduction at 1600 μg/ml of CV extract (IC 50 of CVL for HepG2). Proliferation of WRL68 decreased with a 50% reduction at 1700 μg/ml of CV extract (IC 50 of CV for WRL68). The TUNEL assay showed that CVL induced apoptosis in HepG2 cell line at the concentration of 2000 μg/ml while CV from Japan needed a higher concentration (3000 μg/ml) for apoptosis to occur. The extent of DNA damage by comet assay induced by CVL at 2000 μg/ml was found to be more severe (79% damage) compared to the same concentration of CV from Japan (58% damage). From this study it can be concluded that locally grown CV reduced proliferation and induced apoptosis of liver cancer cell line, HepG2 better than CV from Japan, suggesting that locally produced CV has better anticancer effect on hepatoma cell line.

Item Type:Journal
Additional Information:This research was supported by RM-8 IRPA grant 06- 02-02-0023-PR 0008/09-08 from Ministry of Science, Technology and Innovation (MOSTI). The Chlorella vulgaris from Japan was a kind gift from Prof. Takeshi Hasegawa from Research Laboratories, Chlorella Industry, Chikugo City, Fukuoko, Japan.
Keywords:Chlorella vulgaris, apoptosis, antioxidant, oxidative damage
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2247

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