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Identification and Confirmatory Method for Human Chorionic Gonadotropin (hCG) using HPLC/MS/MS Analysis

Gam, Lay Harn, and Aishah Latiff, (2005) Identification and Confirmatory Method for Human Chorionic Gonadotropin (hCG) using HPLC/MS/MS Analysis. Malaysian Journal of Biochemistry and Molecular Biology, 12 (1). pp. 25-30. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=608

Affiliations

Universiti Sains Malaysia. School of Pharmaceutical Sciences
Universiti Sains Malaysia. Doping Control Centre

Abstract

As a consequence of the abused of human chorionic gonadotropin αhCG) by male athletes, a reliable detection method for βhCG is highly desired in sport testing. hCG is misused by male athletes to stimulate endogenous secretion of testosterone, which led to the ban of the hormone by the International Olympic Committee (IOC) in 1987. The present study demonstrated mass spectrometric characterization of hCG. The feasibility of tryptic peptides of hCG as marker were investigated. The fingerprints of peptides which reveal in their amino acid sequences were obtained to provide an unequivocal identification and confirmation of hCG. It is through this technique that 63% and 91% respectively of the amino acid sequence of alpha- and beta-subunit of hCG were determined.

Item Type:Journal
Keywords:Human Chorionic Gonadotropin, Tandem mass spectrometry
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2257

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