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Genetic Polymorphisms in CYP1A1 (m1), (m2), (m4) and CYP2A6 and Susceptibility to Hepatocellular Carcinoma in a Malaysian Study Population

Suzana Makpol, and Suhana Mamat, and Zalinah Ahmad, and Wan Ngah W.Z., (2005) Genetic Polymorphisms in CYP1A1 (m1), (m2), (m4) and CYP2A6 and Susceptibility to Hepatocellular Carcinoma in a Malaysian Study Population. Malaysian Journal of Biochemistry and Molecular Biology, 12 (1). pp. 31-40. ISSN ISSN 1511-2616

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=609

Affiliations

Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry
Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry
Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry
Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Biochemistry

Abstract

The aim of this study is to investigate the relationship between Cytochrome P450 [CYP1A1 (m1), (m2), (m4) and CYP2A6] genetic polymorphisms and susceptibility to hepatocellular carcinome (HCC) in a population-based case-control study. The frequency of the Cytochrome P450 polymorphisms was determined in 100 HCC patients and compared to frequencies determined in control population. DNA of peripheral white blood cells was isolated and the CYP1A1 (m1), (m2), (m4) and CYP2A6 genotypes were identified as restriction fragment length polymorphisms (RFLPs) by polymerase chain reaction (PCR) and restriction enzyme digests. Our results showed that there was a significant difference in the distribution of CYP1A1 (m2) and CYP2A6 genotypes among Malays, Chinese and Indians in Malaysia (p<0.05). CYP1A1 (m2) genetic polymorphism was found to have a significant relationship with HCC incidence (χ2=31.87, p<0.05) while no significant association between CYP1A1 (m1), (m4) and CYP2A6 genotypes with HCC risk was observed. Odds ratio (OR) and 95% confidence intervals (CI) were calculated by unconditional logistic regression adjusting for known or suspected risk factors for HCC viz. hepatitis B status, family history of cancer, smoking status and alcohol intake. A significant increase in the risk of HCC was observed for individuals with CYP1A1 (m2) mutation (OR=3.69; CI=1.54-8.88). In conclusion, the distribution of CYP1A1 (m2) and CYP2A6 genotypes is different among ethnic groups in Malaysia and variant CYP1A1 (m2) genotype is associated with susceptibility to HCC in Malaysian population.

Item Type:Journal
Keywords:CYP1A1, CYP2A6, polymorphisms, HCC risk
Subjects:Q Science, Computer Science
R Medicine, Dentistry, Pharmacy, Nursing
ID Code:2258

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