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Effect of Carbenicillin on Somatic Embryos Formation of Papaya (Carica papaya L. var. Eksotika I)

Mohamad Fhaizal M. B., and Vilasini, P., and Noorsaadah A. R., and Norzulaani K., (2006) Effect of Carbenicillin on Somatic Embryos Formation of Papaya (Carica papaya L. var. Eksotika I). Malaysian Journal of Science, 25 (2). pp. 47-54. ISSN 13943065

Full text not available from this repository.

Official URL: http://ejum.fsktm.um.edu.my

Affiliations

University of Malaya. Faculty of Science. Inst. of Biological Sciences.
University of Malaya. Faculty of Science. Dept. of Chemistry.
MARDI Headquarters. Strategic, Environment & Natural Resources Research Centre.

Abstract

Embryogenic callus could be established from immature embryo of Carica papaya L. var. Eksotika I after 3 - 4 months of culture on Callus Induction (CI) medium. Embryogenic callus was successfully induced (100 %) on CI medium supplemented with 250 mg/L Carbenicillin plus 10 mg/L 2,4-dichlorophenoxyacetic acids (2,4-D). Transparent somatic embryos then proliferated from the light brown friable embryogenic callus. Carbenicillin that was frequently used as an antibiotic to prevent fungal contamination in in-vitro cultures was found to stimulate the growth of somatic embryos of Carica papaya L. var. Eksotika I. Somatic embryos were maintained in a medium containing either reduced or without 2,4-D. The cultures could be maintained for a period of 5 - 6 months with no apparent loss of regenerative potential. The somatic embryos germinated on Germination (G) medium supplemented with 0.2 mg/L 6-Benzylaminopurine (BAP) and 2.0 mg/L 1-Napthaleneacetic acids (NAA) produced high regeneration frequency (88.41 %) with initial development of hypocotyls followed by rapid growth of plantlets. The in vitro shoots were readily rooted in G medium supplemented with 0.5 mg/L Indole-3-butyric acid (IBA) with 75 % successful rate.

Item Type:Journal
Keywords:Somatic embryogenesis, Carica papaya, carbenicillin
Subjects:S Agriculture
Q Science, Computer Science
ID Code:2382

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