Development of transformation vectors for the production of potentially high oleate transgenic oil palm
Abdul Masani Mat Yunus, and Ahmad Parveez Ghulam Kadir, (2008) Development of transformation vectors for the production of potentially high oleate transgenic oil palm. Electronic Journal of Biotechnology, 11 (3). pp. 1-9. ISSN 07173458 Full text not available from this repository. Official URL: http://www.ejbiotechnology.info/content/vol11/issue3/full/7/7.pdf AffiliationsMalaysian Palm Oil Board. Advanced Biotechnology and Breeding Centre Malaysian Palm Oil Board. Advanced Biotechnology and Breeding Centre AbstractThe main target of Malaysian Palm Oil Board (MPOB)
genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACPthioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors are constructed in this study are suitable for use in both.
particle bombardment (biolistic) and Agrobacteriumbased
transformation protocols. Repository Staff Only: item control page
|