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Biosensor Potentiometrik untuk Penentuan Urea dan Ketoksikan Logam Berat (Potentiometric Biosensor for Determination of Urea and Toxicity of Heavy Metal)

Lee, Yook Heng, and Loh, Han Chern , and Musa Ahmad, (2003) Biosensor Potentiometrik untuk Penentuan Urea dan Ketoksikan Logam Berat (Potentiometric Biosensor for Determination of Urea and Toxicity of Heavy Metal). Sains Malaysiana , 32 . pp. 47-56. ISSN 01266039

Full text not available from this repository.

Official URL: http://pkukmweb.ukm.my/~jsm/english_journals/vol32_2003/vol32_03page47-56.html

Affiliations

Universiti Kebangsaan Malaysia, Faculty of Science and Technology. Centre of Studies on Chemical Sciences and Food Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology. Centre of Studies on Chemical Sciences and Food Technology
Universiti Kebangsaan Malaysia, Faculty of Science and Technology. Centre of Studies on Chemical Sciences and Food Technology

Abstract

A potentiometric biosensor for urea has been designed based on methacrylic­acrylic membranes as matrices for immobilisation. Two types of membranes were used: methacrylic-acrylic membranes with the composition of methyl methacrylate: n-butyl acrylate = 2:8 was used for the immobilisation of a H+ ionophore whilst a polyhydroxyl ethylmethacrylate membrane was employed for the immobilisation of the urease enzyme. By coupling these two mem­branes with a potentiometric transducer, a workable biosensor for the determination of various concentrations of urea, depending on the buffer capacity was designed. The possibility of utilising this biosensor for the detection of heavy metals and analysis of environmental toxicity of metals through inhibition of the urease enzyme was alsoexplored.

Kajian reka bentuk biosensor potentiometrik untuk penentuan urea telah dilakukan dengan menggunakan membran metakrilat-akrilat sebagai matriks pemegunan. Dua jenis membran telah digunakan iaitu membran metakrilat­akrilat dengan komposisi metil metakrilat: n-butil akrilat = 2:8 yang digunakan dalam pemegunan ionofor H+ untuk pengesanan ion hidrogen dan membran polihidroksil etilmetakrilat yang digunakan untuk pemegunan enzim urease. Penggandingan kedua-dua membran ini dengan transduser potentiometrik dapat menghasilkan biosensor urea yang boleh digunakan untuk penentuan urea dalam pelbagai julat kepekatan bergantung kepada muatan larutan penimbal yang digunakan. Di samping itu, kajian terhadap kemungkinan biosensor urea dapat digunakan dalam analisis logam berat dan ketoksikan logam di persekitaran turut dilakukan.

Item Type:Journal
Keywords:Biosensors, Urea, biology
Subjects:Q Science, Computer Science
ID Code:2970

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