Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Histopathological Studies of Cardiac Lesions after an Acute High Dose Administration of Methamphetamine

Kong, Arthur Sn Molh, and Lai, Chin Thing, and Khan, Jesmine, and Al-Jashamy K., and Hasnan Jaafar, and Mohammed Nasimul Islam, (2008) Histopathological Studies of Cardiac Lesions after an Acute High Dose Administration of Methamphetamine. Malaysian Journal of Medical Sciences, 15 (1). pp. 23-30. ISSN 1394195X

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1032Kb

Official URL: http://www.medic.usm.my/publication/mjms/our_journal/mjms_january2008/

Affiliations

Universiti Sains Malaysia, School of Medical Sciences
Universiti Sains Malaysia, School of Health Sciences

Abstract

Eighteen male Wistar rats aged six weeks were divided equally into Methamphetamine (MA), Placebo and Control group. MA group were injected with 50mg/kg body weight of Methamphetamine hydrochloride (MAHCl) in normal saline, Placebo group were injected with normal saline only, while Control group not injected with anything. Five MA group rats died within four hours of injection and their hearts collected on the same day. Another MA group rat was sacrificed two days after injection. Placebo and control group were sacrificed at similar intervals. Collected hearts were studied for cardiac lesions under light microscopy using special staining and immunohistochemistry. Microscopic examination of the myocardium of the rats that died on the first day of injection showed loss of nuclei in some myocytes, indicating cell death. Some areas in the sub-endocardium region showed internalization and enlargement of myocyte nuclei, consistent with regeneration of cells. There were very few foci of necrosis observed in these samples. The heart samples from the single rat that survived injection for two days showed foci of infiltration of macrophage-like cells that were later revealed to be regenerating myocytes. There were also spindle-like fibroblasts, macrophages and a few leucocytes found within these foci. The overall appearance of the myocardium did not indicate any inflammatory response, and the expected signs of necrosis were not observed. These results suggest a need to re-evaluate the toxic and lethal dosages of MA for use in animals testing. Cause of death was suspected to be due to failure of other major organs from acute administration of MA. Death occurred within a time period where significant changes due to necrosis may not be evident in the myocardium. Further investigations of other organs are necessary to help detect death due to acute dosage of MA.

Item Type:Journal
Keywords:MA, acute dose administration, cardiac lesions, myocardium
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3158

1. Derlet RW and Heischober B. Methamphetamine – Stimulant of the 1990s? West J. Med. 1990; 153: 625– 628

2. Jacobs W. Fatal amphetamine-associated cardiotoxicity and its medicolegal implications. Am. Journal of Forensic Medicine & Pathology 2006; 27 (2): 156 – 160

3. Wijetunga M, Seto T, Lindsay J and Schatz I. Crystal methamphetamine-associated cardiomyopathy: Tip of the iceberg? J. of Toxi: Cl. Toxicology 2003; 41 (7): 981 – 986

4. Ellinwood EH, King G and Lee TH. Chronic amphetamine use and abuse. Psychopharmacology: The Fourth Generation of Progress. 2000. Website http://www.acnp.org/G4/GN401000166/CH162.htm, accessed 2.2.2007

5. He SY1, Matoba R, Sodesaki K, Fujitani N and Ito Y. Morphological and morphometric investigation of cardiac lesions after chronic administration of methamphetamine in rats. Nihon Hoigaku Zasshi 1996; 50 (2): 63 – 71

6. Maeno Y, Iwasa M, Inoue H, Koyama H, Matoba R and Nagao M. Direct effects of methamphetamine on hypertrophy and microtubules in cultured adult rat ventricular myocytes. Forensic Science International 2000; 113: 239 – 243

7. Matoba R. Cardiac lesions in methamphetamine abusers. Nihon Hoigaku Zasshi 2001; 55(3): 321 – 330

8. Rostagno C, Caciolli S, Felici M, Gori F and Serneri GGN. Dilated cardiomyopathy associated with chronic consumption of phendimetrazine. Am. Heart J. 1996; 131: 407 – 9

9. Yu Q, Montes S, Larson DF and Watson RR. Effects of chronic methamphetamine exposure on heart function in uninfected and retrovirus-infected mice. Life Sciences 2002; 71: 953 – 965

10. Varner KJ, Ogden BA, Delcarpio J and Meleg-Smith S. Cardiovascular responses elicited by the “binge” administration of methamphetamine. The Journal of Pharmacology and Experimental Therapeutics 2001; 301: 152 – 159

11. Islam MN, Kuroki H, Bai H, Ogura Y, Kawaguchi N, Onishi S and Wakasugi C. Cardiac lesions and their reversibility after long term administration of methamphetamine. Forensic Science International 1995; 75: 29 -43

12. Riviere GJ, Brooks G and Owens M. Disposition of methamphetamine and its metabolite amphetamine in brain and other tissues in rats after intravenous administration. The Journal of Pharmacology and Experimental Therapeutics 2000; 292 (3): 1042 – 1047

13. Shaw KP, Pu CE, Lin DL, Liu JC and Fong JM. A comparative study of inhalation and self-administration methamphetamine-induced toxicities in rats. Advances in Forensic Sciences 1995; 5: 264 – 273

14. Smith HJ, Roche AHG, Jagusch MF and Herdson PB. Cardiomyopathy associated with amphetamine administration. American Heart Journal 1976; 91 (6): 792 – 797

15. Maruta T, Nihira M and Tomita Y. Histopathological study on acute poisoning of methamphetamine, morphine or cocaine. Jpn. J. Alcohol & Drug Dependence 1997; 32 (2): 122 – 138

16. Stefan J and Toupalik P. Our experience with immunocytochemical staining of myoglobin in myocardium. The Journal of Czecho-Slovak Forensic Medicine 1994; 39 (4): 39 – 42

17. Armiger LC and Smeeton WMI. Contraction-band necrosis: Patterns of distribution in the myocardium and their diagnostic usefulness in sudden cardiac death. Pathology 1986; 18: 289 – 295

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.