Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

The Resin-Embedded Cornea Prepared Via Rapid Processing Protocol : A Good Histomorphometric Target For Clinical Investigation In Ophthalmology And Optometry

Cheah, Pike See, and Norhani Mohidin, and Bariah Mohd Ali, and Maung, Myint, and Azian Abdul Latif, (2008) The Resin-Embedded Cornea Prepared Via Rapid Processing Protocol : A Good Histomorphometric Target For Clinical Investigation In Ophthalmology And Optometry. Malaysian Journal of Medical Sciences, 15 (3). pp. 49-54. ISSN 1394195X

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1714Kb

Official URL: http://www.medic.usm.my/publication/mjms/our_journal/mjms_july2008/

Affiliations

Universiti Kebangsaan Malaysia, Faculty of Medicine, Dept. of Anatomy
Universiti Kebangsaan Malaysia, Faculty of Allied Health Sciences, Dept. of Optometry
Universiti Putra Malaysia, Faculty of Medicine & Health Sciences, Dept. of Human Anatomy

Abstract

This study illustrates and quantifies the changes on corneal tissue between the paraffin-embedded and resin-embedded blocks and thus, selects a better target in investigational ophthalmology and optometry via light microscopy. Corneas of two cynomolgus monkeys (Macaca fascicularis) were used in this study. The formalin-fixed cornea was prepared in paraffin block via the conventional tissue processing protocol (4-day protocol) and stained with haematoxylin and eosin. The glutaraldehyde-fixed cornea was prepared in resin block via the rapid and modified tissue processing procedure (1.2-day protocol) and stained with toluidine blue. The paraffin-embedded sample exhibits various undesired tissue damage and artifact such as thinner epithelium (due to the substantial volumic extraction from the tissue), thicker stroma layer (due to the separation of lamellae and the presence of voids) and the distorted endothelium. In contrast, the resin-embedded corneal tissue has demonstrated satisfactory corneal ultrastructural preservation. The rapid and modified tissue processing method for preparing the resin-embedded is particularly beneficial to accelerate the microscopic evaluation in ophthalmology and optometry.

Item Type:Journal
Keywords:Primate, Cornea, Paraffin, Resin
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3380

1. Metha AB, Crane AM, Rylander III HG, Thomsen SL and Albrecht DG. Maintaining the cornea and the general physiological environment in visual neurophysiology experiments. J Neurosci Metho 2001; 109: 153-166.

2. Choo JD, Caroline PJ, Harlin DD and Meyers W. Morphologic changes in cat epithelium following overnight lens wear with the Paragon CRT lens for corneal reshaping. Invest Ophthalmol Vis Sci 2004; 44: E-1552.

3. Matsubara M, Kamei Y, Takeda S, Mukai K, Ishii Y and Ito S. Histologic and histochemical changes in rabbit cornea produced by an orthokeratology lens. Eye Contact Lens 2004; 30: 198-204.

4. Doughty MJ, Bergmanson JPG and Blocker Y. Shrinkage and distortion of the rabbit corneal endothelial cell mosaic caused by a high osmolarity glutaraldehyde-formaldehyde fixative compared to glutaraldehyde. Tissue Cell 1997; 29: 533-547.

5. Doughty MJ. Evidence for heterogeneity in a small squamous cell type (light cells in the rabbit corneal epithelium -a scanning electron microscope study. Doc Ophthalmol 1996; 92: 117-136.

6. Ingris TJJ, Rigby P, Robertson TA, Dutton NS, Henderson M and Chang J. Interaction between Burkholderia pseudomallei and Acanthaamoeba species results in coiling phagocytosis, endamebic bacterial survival, and escape. Infect Immun 2000; 68: 1681-1686.

7. Santhana RL, Cheah, P.S., Nor Asiah C.P., Teh Hamidah Z., Mohidin N., Mohd Ali B., Myint, M. and Azian A.L. Rapid technique: modified protocol for transmission electron microscope visualization of cornea. Ann Microsc 2006; 6: 36-41.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.