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Detection of Immunoglobulin Gene Rearrangement in B-cell Malignancies

Ainoon Othman, and Hamidah Abu Bakar, and Cheong, S.K., and Noor Hamidah Hussin , (2000) Detection of Immunoglobulin Gene Rearrangement in B-cell Malignancies. Malaysian Journal of Pathology, 22 (1). pp. 5-11. ISSN 0126-8635

Full text not available from this repository.

Official URL: http://www.mjpath.org.my/past_issue/MJP2000.1/detection%20of%20immunoglobulin%20gene%20rearrangement%20in%20B%20cell%20malignancies.pdf

Affiliations

Universiti Kebangsaan Malaysia, Hospital UKM. Faculty of Medicine, Dept. of Pathology. Haematology Unit
Universiti Kebangsaan Malaysia, Hospital UKM. Faculty of Medicine, Dept. of Pathology. Haematology Unit
Universiti Kebangsaan Malaysia, Hospital UKM. Faculty of Medicine, Dept. of Pathology. Haematology Unit
Universiti Kebangsaan Malaysia, Hospital UKM. Faculty of Medicine, Dept. of Pathology. Haematology Unit

Abstract

Rearrangement of the immunoglobulin heavy chain (IgH) gene has been used as a marker of lineage and clonality in the diagnosis of B lyrnphoproliferative disorders. A number of PCR-based techniques have been developed to overcome the disadvantages of Southern blotting, the standard technique in detecting IgH gene rearrangement. Using an established seminested PCR technique with consensus primers to the V and J regions of the IgH gene, we analysed DNA prepared from peripheral blood and/or bone marrow specimens from 30 cases of known B cell malignancies (16 chronic lymphocytic leukemia, 11 acute lymphoblastic leukemia and 3 Non-Hodgkin Lymphoma), 3 cases of T lyrnphoproliferative disease and 3 cases of reactive lymphocytosis diagnosed in Hospital UKM
to detect rearranged IgH gene. We found that monoclonality as represented by the presence of rearranged IgH gene were demonstrated in all the 30 cases. The PCR findings showed 100% concordance with the Southern blot analysis results which also showed rearranged IgH bands in all the 30 cases. We also found that none of the cases of T lyrnphoproliferative diseases and reactive lymphocytosis showed presence of rearranged IgH band, suggesting that the amplification using the IgH primers is lineage-specific. In conclusion, we find the PCR a useful method to detect IgH gene rearrangement in peripheral blood and bone marrow specimen. Since the PCR results are comparable to that of the Southern blotting in demonstrating B cell monoclonality and owing to its many advantages we feel that it can replace the Southern blot technique for the diagnosis of B cell malignancies.

Item Type:Journal
Additional Information:This project was fully supported by the IRPA project grant no: 06-02-02-0005 from the Ministry of Science, Technology and the Environment, Malaysia.
Keywords:IgH gene rearrangement, B cell lyrnphoproliferative disorders, DNA methods
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3401

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