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Physiological responses of Escherichia coli cells cultivated under a sublethal oxidative stress condition

Krishnaiah, D., and Bono, A., and Sarbatly, R., and Nishioka, M., and Kim, S. Y., and Tanaka, M., and Ojima, Y, and Taya, M, (2007) Physiological responses of Escherichia coli cells cultivated under a sublethal oxidative stress condition. Malaysian Journal of Microbiology , 3 (1). pp. 14-18. ISSN 9834240708

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Official URL: http://www.usm.my/mjm/

Affiliations

Universiti Malaysia Sabah. School of Engineering and Information Technologyl. Chemical Engineering Programme
Universiti Malaysia Sabah. School of Engineering and Information Technologyl. Chemical Engineering Programme
Universiti Malaysia Sabah. School of Engineering and Information Technologyl. Chemical Engineering Programme
Osaka University. Graduate School of Engineering Science. Dept. of Materials Engineering Science. 2Division of Chemical Engineering
Osaka University. Graduate School of Engineering Science. Dept. of Materials Engineering Science. 2Division of Chemical Engineering
Osaka University. Graduate School of Engineering Science. Dept. of Materials Engineering Science. 2Division of Chemical Engineering
Osaka University. Graduate School of Engineering Science. Dept. of Materials Engineering Science. 2Division of Chemical Engineering

Abstract

Superoxide dismutase (SOD) is widely distributed in organisms and alleviates toxicity of reactive oxygen species (ROS) formed inside cells. It was found that sublethal oxidative stress derived from photoexcited TiO2 exerted a simulative effect on the growth of SOD null mutant of Escherichia coli (IM303) with reduction in intracellular ROS level. DNA microarray analysis was then carried out to compare gene expression between IM303 cells with and without the oxidative stress. From the DNA micro array data, yfiD, yggB and yggE were selected as genes up- regulated under the oxidative condition and then cloned into a pUC 19 plasmid. The original pUC 19 and constructed plasmids were introduced into E. coli MM294 and the transformants were cultivated in M9 medium with paraquat. Among these transformants, intracellular ROS content was the lowest in the cells carrying yggE gene and maximum specific growth rate of those cells was also higher than that of control cells with pUC 19. These results suggest that yggE gene product has an ROS-scavenging function in the cells of E. coli cells exposed to an oxidative stress and improves the efficiency of cellular growth.

Item Type:Journal
Keywords:Escherichia coli, reactive oxidative stress, cell cultivation, photoexcited TiO2.
Subjects:Q Science, Computer Science
T Technology, Engineering
ID Code:3523

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