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Isolation of Human Surfactant Protein A from Amniotic Fluid

Cheong, K.B., and Cheong, S.,K., and Boo, N.Y., and Jemilah M, and Ton, S.H., (1995) Isolation of Human Surfactant Protein A from Amniotic Fluid. Malaysian Journal of Pathology, 17 (2). pp. 97-101. ISSN 0126-8635

Full text not available from this repository.

Official URL: http://www.mjpath.org.my/past_issue/MJP1995.2/isolation%20of%20human%20surfactant%20protein%20A%20from%20amniotic%20fluid.pdf

Affiliations

Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Paediatrics["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Obstetrics and Gynecology["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Biochemistry

Abstract

Surfactant protein A (SP-A) is one of the four known surfactant-associated proteins found in human lungs. It plays a major role in determining regulation of surfactant uptake and resecretion. Qualitative and quantitative deficiencies of SP-A may contribute to neonatal respiratory distress syndrome. The measurement of its level in amniotic fluid or neonatal tracheal aspirate may be useful in the assessment of replacement therapy using natural or synthetic surfactants. In order to develop an in-house immunoassay to detect the level of SP-A, we used a discontinuous sucrose density gradient to isolate SP-A from amniotic fluid. Polyacrylamide gel electrophoresis was carried out on the isolates with low molecular weight markers. We successfully isolated SP-A from 12 out of 31 samples of amniotic fluid. The isolates were found to be relatively pure and have a molecular weight of about 35 kD. The isolated SP-A were used as immunogens to raise antibodies in rabbits for the immunoassay .

Item Type:Journal
Additional Information:This note was added by the search_and_modify.pl script.
Keywords:SP-A, respiratory distress syndrome, polyacrylamide gel electrophoresis, sucrose density gradient.
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3589

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