Evaluating In-house Anti-serum Against B Cells With Flow Cytometry
Chin, S.F., and Cheong, S.K., and Lim, Y.C., and Mok, K.L., and Hamidah Hussin Noor, (1993) Evaluating In-house Anti-serum Against B Cells With Flow Cytometry. Malaysian Journal of Pathology, 15 (2). pp. 125-130. ISSN 0126-8635 Full text not available from this repository. Official URL: http://www.mjpath.org.my/past_issue/MJP1993.2/evaluating%20in%20house%20antiserum%20against%20B%20cells%20with%20flow%20cytometry.pdf AffiliationsUniversiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology. Haematology Unit["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology. Haematology Unit["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology. Haematology Unit["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology. Haematology Unit["lib/metafield:join_corp_creators" not defined]Universiti Kebangsaan Malaysia, Faculty of Medicine. Dept. of Pathology. Haematology Unit AbstractThe applications of antibodies, be it monoclonal or polyclonal, in the diagnostic and research fields are well established. The disadvantage is the high cost of commercially available antibodies. In a diagnostic establishment like ours which also funclions as a training ground for laboratory related personnel, it is beneficial to be able to produce in-house reagents. Therefore, we have undertaken this project to produce a rabbit polyclonal antibody against B lymphocytes. We found that the rabbit was a good choice because the titre of antibody produced was high and positive reactions were still detected at a dilution of 1:38400. The antibody showed significant positive reaction only with the lymphocyte subpopulation. A positive reaction was observed between the immunized rabbit serum and B lyinphocy Les but 1101 T lymphocytes. This shows that the antibody was B lyinphocytc specific. There was a positive correlation bctwcc~l thc pcrccntage of t3 lyrnphocyres labelled using Lhe commercial anti-CD L9 monoclona antibody and Ihc in-housc polyclonal antibody (n= 13, r=0.7, p=0.02). However. the percentage of cells labelled by ihe in-house polyclonal anti-B ivas lower than that by the commercial n~onoclonal anti-CDl9. The fluorescence intensity of the polyclonal antibody was lower than that of the monoclonal. In general, the performance of the in-house polyclonnl antibody can be considered as satisfactory. The rabbi1 serum was stored at -2W and no significarit loss of activity was detected tor over a period of 19 months. Kcj ~ ~ u r dPsn:l yclonal antibody, B lymphocytes, phenotyping, flow cytometry. The fluorescence intensity of the polyclonal antibody was lower than that of the monoclonal. In general, the performance of the in-house polyclonnl antibody can be considered as satisfactory. The rabbit serum was stored at -2W and no significarit loss of activity was detected tor over a period of 19 months. | Item Type: | Journal |
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| Additional Information: | This note was added by the search_and_modify.pl script. |
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| Keywords: | Polyyclonal antibody, B lymphocytes, phenotyping, flow cytometry. |
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| Subjects: | R Medicine, Dentistry, Pharmacy, Nursing |
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| ID Code: | 3622 |
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