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Immunolabelling of Prolactin at Ultrastructural Level Using the Protein A-gold Technique on Epon-embedded Tissue

Ng, K.K., and Tan, Kirn-Lian, and Gan, S.K., and Looi, L.M., (1992) Immunolabelling of Prolactin at Ultrastructural Level Using the Protein A-gold Technique on Epon-embedded Tissue. Malaysian Journal of Pathology, 14 (1). pp. 29-33. ISSN 0126-8635

Full text not available from this repository.

Official URL: http://www.mjpath.org.my/past_issue/MJP1992.1/immunolabelling%20of%20prolactin%20at%20ultrastructural%20level%20using%20the%20protein.pdf

Affiliations

University of Malaya, Faculty of Medicine. Dept. of Radiology["lib/metafield:join_corp_creators" not defined]University of Malaya, Faculty of Medicine. Dept. of Pathology ["lib/metafield:join_corp_creators" not defined]University of Malaya, Faculty of Medicine. Electron Microscopy Unit["lib/metafield:join_corp_creators" not defined]University of Malaya, Faculty of Medicine. Dept. of Pathology

Abstract

The use of the colloidal-gold technique in electron microscopy immunocytochemistry has provided important information on the in situ localisation of intracellular antigens. We have developed a postembedding technique for prolactin localisation on resin-embedded human pituitary tissue sections by the use of the protein-A gold conjugate. Human pituitary tissue obtained at autopsy was processed for electron microscopical study without post-osmication and then embedded in Epon. The indirect immunoperoxidase method was used for light microscopical targetting of lactotroph cells for subsequent electron microscopical antigen localisation. Ultra-thin sections were labelled with human anti-human prolactin followed by protein-A gold conjugate. Specific labelling was observed over secretory granules with a density of 15-30 particles per granule, as determined by the Quantimet 570 image analysis system. This technique provides a means of studying the pathophysiology of hormonal secretion at ultrastructural level and can be a useful tool in diagnostic and research investigations.

Item Type:Journal
Additional Information:This note was added by the search_and_modify.pl script.
Keywords:Protein A-Gold, immunolabelling, prolactin, antigen localisation
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3642

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