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Use of Dengue Blot in Dengue Diagnosis: The Malaysian Experience

Fang, R., and Sinniah, M., and Kuen, L.S., (1992) Use of Dengue Blot in Dengue Diagnosis: The Malaysian Experience. Malaysian Journal of Pathology, 14 (2). pp. 117-120. ISSN 0126-8635

Full text not available from this repository.

Official URL: http://www.mjpath.org.my/past_issue/MJP1992.2/use%20of%20dengue%20blot%20in%20dengue%20diagnosis.pdf

Affiliations

Institute for Medical Research Malaysia. Division of Virology["lib/metafield:join_corp_creators" not defined]Institute for Medical Research Malaysia. Division of Virology["lib/metafield:join_corp_creators" not defined]Institute for Medical Research Malaysia. Division of Virology

Abstract

Dengue feverpengue haemorrhagic fever (DFPHF) has been a public health problem in Malaysia with an endemic level of about 7 per 100,000 population per year. In 1990, Malaysia experienced its most severe outbreak of DFPHF with a record total of 5,590 cases referred to the Division of Virology, Institute for Medical Research (IMR). Of these, 1,880 were confirmed serologically to be DFPHF. The conventional serological procedure, the Haemagglutination Inhibition (HI) test, for the diagnosis of DFPHF is cumbersome and causes delay in diagnosis. Another problem associated with the HI test has been that it has often been difficult to obtain a second convalescent serum sample for an accurate diagnosis. This has raised an urgent need to establish a "rapid" test for diagnosis of DFPHF. As such the authors recently carried out an evaluation of a newly available commercial rapid test, namely, the Dengue Blot Assay (Diagnostic Biotechnology Singapore Pte Ltd). The test is intended for use in laboratory confirmation of dengue virus infection. The evaluation was to determine if the test could be utilised as a routine laboratory test and to establish its sensitivity and specificity. Over 400 samples were tested against the Dengue Blot Assay. Results were checked against an in-house Dengue 1gM ELISA and HI assay. Preliminary results indicate that the sensitivity and specificity of the Dengue Blot is satisfactory. Our results also indicate that the Dengue Blot has a useful role to play in a routine laboratory especially since it provides rapid results on single serum samples thereby reducing the workload in a busy diagnostic laboratory.

Item Type:Journal
Additional Information:This note was added by the search_and_modify.pl script.
Keywords:Dengue, technology, evaluation
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3707

1. Institute for Medical Research, Annual Report, 1991, IMR, Kuala Lumpur, Malaysia.

2. Clarke DH, Casals J. Techniques for haemagglutination and haemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 1958; 7: 561-73.

3. Lam SK, Devi S, Pang T. Detection of specific IgM in dengue infection. Southeast Asian J Trop Med Pub Hlth 1987; 18 (4): 532-7.

4. Technical Advisory Group on Dengue Haemorrhagic FeverPengue Shock Syndrome. Dengue Haernorrhagic Fever Diagnosis, Treatment and Control, 1986; World Health Organisation, Geneva, Switzerland.

5. Lim TW. Laboratory diagnosis of dengue. In: Rudnick A, Lim TW, Eds: Dengue Fever Studies in Malaysia, Bulletin No.23, IMR, 1986: 169-80.

6. Lai OF, Chan YC, Ngoh BL, Tan HC, Chan L. tional Congress of Virology, Berlin, August 26 - 31, 1990.

7. Chan YC, Lai OF, Ngoh BL, Tan HC, Chan L. Dengue diagnosis using a commercial kit (Dengue Blot): a prospective study. Proceedings of the VIII International Congress of Virology, Berlin, August 26-3 1, 1990.

8. Cardosa MJ. Diagnosis and surveillance of dengue virus infections: gold Standards and new directions. Malaysian Journal of Pathology 1989; 11: 7- 10. Rapid diagnosis of dengue by a commercial kit (Dengue Blot). Proceedings of the VIII Intema

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