Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Ammonium Uptake and Growth Kinetics of Pseudomonas putida PGA1

Annuar M.S.M, and Tan, I.K.P., and Ibrahim S., and Ramachandran, K.B., (2006) Ammonium Uptake and Growth Kinetics of Pseudomonas putida PGA1. Asia Pacific Journal of Molecular Biology & Biotechnology, 14 (1). pp. 1-10.

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
373Kb

Official URL: http://www.msmbb.org.my/apjmbb/html141/msma.pdf

Affiliations

University of Malaya. Faculty of Science. Institute of Biological Sciences
University of Malaya. Faculty of Science. Institute of Biological Sciences
University of Malaya. Faculty of Engineering. Dept. of Civil Engineering
Indian Institute of Technology. Dept. of Biotechnology

Abstract

Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gramnegative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the micro-organism’s specific uptake rate of ammonium and its growth would increase as the ammonium ion concentrations become higher. Optimal ammonium uptake and growth rates were observed at 0.1 gL-1 ammonium ion in aqueous medium. It also indicated that the ammonium transport mechanism is probably inducible by ammonium concentration. The limit of ammonium ion level increase before cells stop growing completely is given by the substrate inhibition constant, Ki which equals to 1.2 gL-1 ammonium ion concentration.

Item Type:Journal
Additional Information:This research was funded by the University of Malaya (grant no. Vot F0156/2001A).
Keywords:Ammonium, growth, kinetics, uptake, poly-(3-hydroxyalkanoates)
Subjects:Q Science, Computer Science
ID Code:3810

Atkinson, B. and Mavituna, F. 1991. Biochemical Engineering

and Biotechnology Handbook. 2nd Edition: MacMillan Publishers Ltd. pp.120.

Beaulieu, M., Beaulieu, Y., Melinard, J., Pandian, S. and Goulet, J. 1995. Influence of ammonium salts and cane molasses on growth of Alcaligenes eutrophus and production of polyhydroxybutyrate. Applied and Environmental Microbiology 61: 165-169.

Belfares, L., Perrier, M., Ramsay, B.A., Ramsay, J.A., Jolicoeur, M. and Chavarie, C. 1995. Multi-inhibition kinetic model for the growth of Alcaligenes eutrophus. Canadian Journal of Microbiology 41: 249-256.

Bitar, A. and Underhill, S. 1990. Effect of ammonium supplementation on production of poly-bhydroxybutyric acid by Alcaligenes eutrophus in batch culture. Biotechnology Letters 12: 563-568.

Burkovski, A. 2003. I do it my way: regulation of ammonium uptake and ammonium assimilation in Corynebacterium glutamicum. Archive of Microbiolology 179: 83-88.

Cunningham, L. and Williams, H.D. 1995. Isolation and characterization of mutants defective in the cyanideinsensitive respiratory pathway of Pseudomonas aeruginosa. Journal of Bacteriology 177: 432-438.

Gunstone, F.D., Harwood, J.L. and Padley, F.B. 1994. The Lipid Handbook. 2nd Edition: Chapman and Hall Chemical Database pp.330.

Hazenberg, W and Witholt, B. 1997. Efficient production of medium-chain-length poly(3-hydroxyalkanoates) from octane by Pseudomonas oleovorans : economic considerations. Applied Microbiology and Biotechnology 48: 588-596.

Hocking, P.J. and Marchessault, R.H. 1994. Biopolyesters. In Chemistry and Technology of Biodegradable Polymers. ed. Griffin, G.S.L. pp. 48-96: Blackie Academic and Professional, Chapman & Hall.

Huisman, G.W., de Leeuw, O., Eggink, G. and Witholt, B. 1989. Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. Applied and Environmental Microbiology 55: 1949-1954.

Jahns, T., Kaltwasser, H. and Kleiner, D. 1986 Ammonium methylammonium) uptake by Alcaligenes eutrophus H16. Archives of Microbiology 145: 306-310.

Kim, G.J, Lee, I.Y, Yoon, S.C, Shin, Y.C, Prak, Y.H.1997. Enhanced yield and high production of mediumchain-

length poly(3-hydroxyalkanoates) in a twostep fed-batch cultivation of Pseudomonas putida by combined use of glucose and octanoate. Enzyme and Microbial Technology 20: 500-505.

Kleiner, D. 1985. Bacterial ammonium transport. FEMS Microbiology Reviews 32: 87-100.

Lageveen, R.G, Huisman, G.W, Preusting, H, Ketelaar, P, Eggink, G, Witholt, B. 1988. Formation of polyesters by Pseudomonas oleovorans: effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Applied and Environmental Microbiology 54: 2924-2932.

Madison, L. and Huisman, G.W. 1999. Metabolic engineering of poly(3-hydroxyalkanoates): From DNA to plastic.Microbiology and Molecular Biology Reviews 63:21-53.

Mulchandani, A., Luong, J.H.T. and Groom, C. 1989. Substrate inhibition kinetics for microbial growth and synthesis of poly-b-hydroxybutyric acid by Alcaligenes eutrophus ATCC 17697. Applied Microbiology and Biotechnology 30: 11-17.

Russell, J.B. and Cook, G.M. 1995. Energetics of bacterial growth: balance of anabolic and catabolic reactions. Microbiological Reviews 59: 48-62.

Shuler, M.L. and Kargi, F. 1992. Bioprocess Engineering: Basic Concepts: Prentice-Hall International, Inc. pp. 199-209.

Solorzano, L. 1969. Determination of ammonia in natural waters by the phenolhypochlorite method. Limnology and Oceanography 14: 799-801.

Suzuki, T., Yamane, T. and Shimizu, S. 1986. Mass production of poly-b-hydroxybutyric acid by fully automatic fedbatch culture of methylotroph. Applied Microbiology and Biotechnology 23: 322-329.

Tan, I.K.P, K.Sudesh Kumar, Theanmalar, M, Gan, S.N, B.Gordon III 1997. Saponified palm kernel oil and its major free fatty acids as carbon substrates for the production of polyhydroxyalkanoates in Pseudomonas putida PGA1. Applied Microbiology and Biotechnology 47:207-211.

Terpe, K., Kerkhoff, K., Pluta, E. and Jendrossek, D. 1999. Relationship between succinate transport and production of extracellular poly(3-hydroxybutyrate) depolymerase in Pseudomonas lemoignei. Applied and Environmental Microbiology 65: 1703-1709.

Wang, J.G. and Bakken, L.R. 1998. Screening of soil bacteria for poly-b-hydroxybutyric acid production and its role in the survival of starvation. Microbial Ecology 35: 94101.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.