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Negative Cross-reactivity of Rabbit anti-Malassezia Furfur Antibodies with Other Yeasts

Chua, Kaw Bing , and Devi, Shamala, and Ng, Kee Peng , and Hooi, Poh Sim, and Na, Shiang Ling , and Chua, Kerk Hsiang , (2005) Negative Cross-reactivity of Rabbit anti-Malassezia Furfur Antibodies with Other Yeasts. Malaysian Journal of Pathology, 27 (2). pp. 123-125. ISSN 0126-8635

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Official URL: http://www.mjpath.org.my/past_issue/MJP2005.2/09Short.pdf

Affiliations

International Medical University, Kuala Lumpur,
University of Malaya, Faculty of Medicine. Dept. of Medical Microbiology,
University of Malaya, Faculty of Medicine. Dept. of Medical Microbiology,
University of Malaya, Faculty of Medicine. Dept. of Medical Microbiology,
University of Malaya, Faculty of Medicine. Dept. of Medical Microbiology,
Adelaide University, Australia

Abstract

Anti-Malassezia furfur monospecific polyclonal antibodies was produced by repeated immunization of rabbit with Malassezia furfur yeast cells mixed with Freud adjuvant. The antibody titres of respective rabbit’s serum samples prior to and after each immunization against M. furfur were assayed by indirect immunofluorescence technique using the M. furfur whole yeast antigen fixed in Teflon coated slides. The highest anti-M. furfur antibody titre achieved was 1 in 1280 dilution. At 1:20 dilution, none of the respective serum samples taken at various stages of immunization gave positive immunofluorescent staining against any of the other species of yeasts tested in this study. Anti-M. furfur monospecific polyclonal antibodies produced in rabbit in this study has the potential for diagnostic application in immunohistochemical detection of M. furfur in human tissues.

Item Type:Journal
Additional Information:We thank Mr Chua Kiew Heng, medical laboratory technologist of Melaka General Hospital, for his financial and moral support in this project. We are grateful to Dr Soo-Hoo Tuck Soon, Associate Professor of Mycology, Department of Medical Microbiology, University of Malaya for the generous gift of the other species of mycological yeasts.
Keywords:Malassezia furfur, antibodies, immunofluorescence.
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:3828

1. Kwon-Chung KJ, Bennett JE. Infections caused by Malassezia species. In: Medical Mycology. Publisher: Lea & Febiger, Philadelphia. London 1992; chapter 8: pp 170-182.

2. Midgley G. The diversity of Pityrosporum (Malassezia)

yeasts in vivo and in vitro. Mycopathologia 1989; 106: 143-53.

3. Nakabayashi A, Sei Y, Guillot J. Identification of Malassezia species isolated from patients with seborroeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects. Med Mycol 2000; 38: 337-41.

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