The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract
Tau, Chuan Ling, and Lyddiatt, Andrew, (2005) The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract. Asia Pacific Journal of Molecular Biology & Biotechnology, 13 (1). pp. 59-62.
Official URL: http://www.msmbb.org.my/apjmbb/html13/tau2.pdf
Universiti Putra Malaysia. Faculty of Engineering. Department of Process and Food Engineering
Millipore Biopharmaceutical Division, Bioprocessing Ltd, United Kingdom
The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity.
|Additional Information:||Tau Chuan Ling gratefully acknowledges financial support
from Universiti Putra Malaysia, Malaysia and the School of
Chemical Engineering, University of Birmingham, UK.|
|Keywords:||anion exchange, dye-ligand, Cibacron Blue 3GA, fluidised bed adsorption, G3PDH|
|Subjects:||Q Science, Computer Science|
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