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The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract

Tau, Chuan Ling, and Lyddiatt, Andrew, (2005) The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract. Asia Pacific Journal of Molecular Biology & Biotechnology, 13 (1). pp. 59-62.

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Affiliations

Universiti Putra Malaysia. Faculty of Engineering. Department of Process and Food Engineering
Millipore Biopharmaceutical Division, Bioprocessing Ltd, United Kingdom

Abstract

The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity.

Item Type:Journal
Additional Information:Tau Chuan Ling gratefully acknowledges financial support from Universiti Putra Malaysia, Malaysia and the School of Chemical Engineering, University of Birmingham, UK.
Keywords:anion exchange, dye-ligand, Cibacron Blue 3GA, fluidised bed adsorption, G3PDH
Subjects:Q Science, Computer Science
ID Code:3884

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