Rapid Production of Lilium auratum Bulbs from Zygotic Embryos
Furyuya, Takuo, and Nomura, Koji, (2004) Rapid Production of Lilium auratum Bulbs from Zygotic Embryos. Asia Pacific Journal of Molecular Biology & Biotechnology, 12 (1 & 2). pp. 39-42.
Official URL: http://www.msmbb.org.my/apjmbb/html12/123942.pdf
Ibaraki Prefectural Sashima Senior High School, Japan
University of Tsukuba, Japan. Institute of Agriculture and Forestry
An in vitro culture system for Lilium auratum Lindl. zygotic embryos was established to accelerate the development of bulblets. High rates of germination were observed on 50 days after pollination (DAP) or later, while the rate of normal growth increased after 70 DAP. Peeling of the seed coats was necessary for rapid in vitro germination, indicating inhibition by the seed coats. We found that embryos obtained from 90 DAP capsules were the best materials for suppressing dormancy. Further development of bulblets was accelerated by culturing them in the presence of 9 % sucrose. In this culture system, the period for development of mature bulblets was reduced from a couple of years to one year after pollination.
|Keywords:||Lilium auratum Lindl, ovule culture, embryo culture, propagation, genetic diversity|
|Subjects:||Q Science, Computer Science|
Repository Staff Only: item control page