Molecular Subtyping of Clinical Isolates Vibrio cholerae from Malaysia and Vietnam by Pulsed-field Gel Electrophoresis

Thong, Kwai Lin, and Goh, Yee Ling, and Phung, Dac Cam, (2002) Molecular Subtyping of Clinical Isolates Vibrio cholerae from Malaysia and Vietnam by Pulsed-field Gel Electrophoresis. Asia Pacific Journal of Molecular Biology & Biotechnology, 10 (2). pp. 103-108.

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Affiliations

University of Malaya. Faculty of Science. Institute of Biological Science
niversity of Malaya. Faculty of Science. Institute of Biological Science
National Institute of Hygiene and Epidemiology, Hanoi, Vietnam, Dept of Microbiology

Abstract

Pulsed-field gel electrophoresis (PFGE) was applied to analyse sporadic and outbreak cases of cholera caused by V. cholerae O1 biotype El Tor from Vietnam (n=16) and V. cholerae O139 from Malaysia (n=12) and from five vaccine strains. NotI-Digestion of chromosomal DNA from these isolates, followed by PFGE produced eleven different profiles among the 16 V. cholerae O1 isolates (Dice coefficient: 0.67-0.97). The V. cholerae O1 Ogawa and Inaba strains were clearly differentiated into distinct clusters. NotI-PFGE analysis among V. cholerae O139 isolates showed a predominant PFGE profile (N12) indicating that the outbreak was probably derived a single clone. Among the five vaccine strains, the PFGE profiles of V. cholerae Inaba Cairo 48 and classical Inaba Cairo 48 were similar to some of the field isolates of 01 Inaba strains in Vietnam. The PFGE analysis showed that the V. cholerae 0139 isolates from Malaysia were indistinguishable as these strains were probably derived from a cholera outbreak and the V. cholerae O1 isolates from Vietnam exhibited limited genetic diversity.

Item Type:

Journal

Keywords:

Pulsed-field gel electrophoresis , PFGE, Vibrio cholerae O1 and O139

Subjects:

Q Science, Computer Science

ID Code:

4062