Influence of Irradiance and Inoculum Density on the Pigmentation of Spirulina platensis
Chu, Wan Loy, and Phang, Siew-Moi, and Miyakawa, K,, and Tosu, K., (2002) Influence of Irradiance and Inoculum Density on the Pigmentation of Spirulina platensis. Asia Pacific Journal of Molecular Biology & Biotechnology, 10 (2). pp. 109-117.
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Official URL: http://www.msmbb.org.my/apjmbb/html102/102109.htm
International Medical University Malaysia
University of Malaya. Faculty of Science. Institute of Biological Science
Dainippon Ink & Chemicals Inc., Food and Food Additives Division, Japan
Siam Algae Co. Ltd, Thailand
The effects of irradiance and inoculum density on the growth and pigmentation, especially carotenoid composition of Spirulina (Arthospira) platensis UMACC 160 were investigated. The cultures were grown at three irradiance levels (50, 105 and 160 µmol m-2 s-1) using two inoculum densities (OD620= 0.2 and 2.0). Biomass and pigment contents throughout the growth cycle were determined. The low-density inoculum cultures attained higher specific growth rates (µ) based on dry weight (0.54 day-1) than the high-density inoculum cultures (0.26 day-1). For the low-density inoculum cultures, specific growth rate (µ) increased with increasing irradiance. Maximum biomass attained under the various culture conditions reached 1363 mg dry weight L-1. The contents of phycocyanin, chlorophyll a and carotenoids varied markedly, ranging from 1.1 - 127.0, 7.2 - 32.2 and 3.30 - 12.19 mg g-1 dry weight respectively. The highest yield of phycocyanin (919 mg L-1) was attained by the low-density inoculum cultures grown at the lowest irradiance, while the highest yield of carotenoids (8.50 mg L-1) was attained by the high-density inoculum cultures grown at the highest irradiance, both at stationary phase. The carotenoids consisted of β-carotene (5.50 mg g-1 dry weight), myxoxanthophyll (4.62 mg g-1 dry weight), zeaxanthin (2.52 mg g-1 dry weight) and oscillaxanthin (1.14 mg g-1 dry weight); the content of each constituent varied with culture age and irradiance, and was dependent on the inoculum density used. Irradiance, inoculum density and culture age are three important factors to be considered for optimisation of pigment production by S. platensis.
|Keywords:||β-carotene, carotenoids, chlorophyll a, irradiance, phycocyanin, Spirulina platensis \|
|Subjects:||Q Science, Computer Science|
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