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Prevalence and viability assessment of endophytic fungi in Iranian tall fescue (Festuca arundinacea Schreb.)

Nekouei, M. Khayyam, and Mirlohi. A.F., and Shahab, M. Naderi, and Sariah Meon, and Manaf Ali A., (2001) Prevalence and viability assessment of endophytic fungi in Iranian tall fescue (Festuca arundinacea Schreb.). Asia Pacific Journal of Molecular Biology & Biotechnology, 9 (1). pp. 60-66.

Full text not available from this repository.

Official URL: http://www.msmbb.org.my/apjmbb/html91/9160.htm

Affiliations

Universiti Putra Malaysia. Faculty of Food Science and Biotechnology. Dept. of Biotechnology
Isfahan University of Technology, Iran. College of Agriculture. Dept. of Agronomy and Plant Breeding
Research Institute of Forests and Rangelands Tehran, Iran. Dept. of Genetic and Plant Physiology
Universiti Putra Malaysia. Faculty of Agriculture. Dept. of Plant Protection
Universiti Putra Malaysia. Faculty of Food Science and Biotechnology. Dept. of Biotechnology

Abstract

An assessment on the infection status and viability of endophytic fungi in Iranian tall fescue would aid in identifying potentially desirable endophyte strains for use in genetic improvement of pasture and turf species. Nineteen tall fescue accessions collected from various regions were screened for Neotyphodium infection. Fifty seeds per accession were evaluated by microscopic staining methods to detect the presence of endophyte(s), and also to determine the infection rate in the collected tall fescue accessions. Out of the 19 accessions evaluated, 16 (84.2%) were found to be infected with endophyte(s) at infection rates of between 20 to 95%. Out of those infected, five accessions recorded infection rates of more than 90% while 3 accessions were free of endophytes. The occurrence of endophytic fungi were also determined in seedlings as an indication of fungal viability. Fifty tillers per accession were microscopically examined; and out of the 16 infected seed accessions analysed, 14 had viable endophytes in the seedlings. The incidence of endophytic fungi infection, based on the percentages of infection in seedlings, varied from 15% to 100%. The average rate of infection marginally decreased from seeds to seedlings suggesting the reduction in fungal viability upon seed storage. To our knowledge, this is the first report on the presence of endophytic fungi in Iranian tall fescue germplasm c61lection and consequently the germplasm collection can provide a greater diversity of plant and fungus genotypes for introduction into future plant breeding programs.

Item Type:Journal
Keywords:Endophytic fungi, Neotyphodium sp., Acremonium sp., tall fescue, Festuca arundinacea, symbiosis
Subjects:Q Science, Computer Science
ID Code:4089

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