Abundance of random amplified hybridising microsatellites in mungbean (Vigna radiata L.Wilczek)

Prasad, S., and Reddy, K.S., and Jawali, N., (1999) Abundance of random amplified hybridising microsatellites in mungbean (Vigna radiata L.Wilczek). Asia Pacific Journal of Molecular Biology & Biotechnology, 7 (2). pp. 173-178.

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Affiliations

Bhabha Atomic Research Centre, Trombay, Mumbai, India. Molecular Biology and Agriculture Division["lib/metafield:join_corp_creators" not defined]Bhabha Atomic Research Centre, Trombay, Mumbai, India. Nuclear Agriculture and Biotechnology Division["lib/metafield:join_corp_creators" not defined]Bhabha Atomic Research Centre, Trombay, Mumbai, India. Molecular Biology and Agriculture Division

Abstract

In eukaryote genomes microsatellites are abundant and dispersed and they have gained importance as single locus markers because of their high level of polymorphism. The abundance of some tri and tetranuclcotide repeat microsatellites were surveyed in random amplified DNA fragments for the development of microsatellite markers for mungbean. Amplification products obtained from 39 arbitrary primers were screened. The data obtained on random amplified products hybridising microsatellites (RAHMs) show GGTT repeat RAHMs is the most abundant followed by CAA and CAC repeat RAHMs. The tetranucleoticle GCGT repeat RAHMs constitute nearly half as much as CAA repeat RAHMs, whilst GACA repeat RAHMs are absent. Some primer/ probe combinations were used for assessing RAHM polymorphism among 15 genotypes of mungbean. The polymorphic RAHMs (Random Amplified Microsatellite Polymorphism :RAMPO) were essentially dominant in nature (as in the case of RAPD).

Item Type:

Journal

Additional Information:

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Keywords:

Arbitrary primers, microsatellites, mungbean, RAHM, RAMPO

Subjects:

Q Science, Computer Science

ID Code:

4131