Synergistic effects of medium strategies and sucrose levels with nitrogenous compounds, phosphate and myo inositol on Morinda elliptica cell cultures

Abdullah M.A., and Marziah M., and Zakaria M.N., and Ali A.M., and Lajis N.H., and Ariff A.B., (1999) Synergistic effects of medium strategies and sucrose levels with nitrogenous compounds, phosphate and myo inositol on Morinda elliptica cell cultures. Asia Pacific Journal of Molecular Biology & Biotechnology, 7 (1). pp. 61-72.

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Affiliations

Universiti Putra Malaysia. Dept. of Biotechnology["lib/metafield:join_corp_creators" not defined]Universiti Putra Malaysia. Dept. of Biochemistry and Microbiology["lib/metafield:join_corp_creators" not defined]Universiti Putra Malaysia. Dept. of Biotechnology["lib/metafield:join_corp_creators" not defined]Universiti Putra Malaysia. Dept. of Biotechnology["lib/metafield:join_corp_creators" not defined]Universiti Putra Malaysia. Dept. of Chemistry["lib/metafield:join_corp_creators" not defined]Universiti Putra Malaysia. Dept. of Biotechnology

Abstract

Synergistic effects of medium strategies and sucrose levels with nitrogenous compounds, phosphate and myo inositol on Morinda elliptica cell suspension cultures were studied. Three types of medium strategies were tested maintenance (M), growth (G) and production (P) media. In M medium, incorporation of NO3 between 30 mM and 67.7 mM, was vital for optimum growth and anthraquinone (AQ) production. NO3 level upto 95.9 mM did not affect cell growth though AQ may be slightly reduced. At NO3 level of 77.1 mM, 8% (w/v) sucrose in G medium produced optimum cell growth and AQ content but cell growth was reduced whilst AQ content unaffected in P medium. At 10% (w/v) sucrose in both G and P media, both cell growth and AQ content were reduced. Phosphate was more a growth limiting nutrient than an AQ limiting nutrient. Absence of phosphate retarded cell growth in M medium but no significant difference on cell growth and AQ yield was observed with 5 fold increase in phosphate in M medium; at 8% (w/v) and 10% (w/v) sucrose in G medium; and at 8% (w/v) sucrose in P medium. At 10%(w/v) sucrose in P medium, the AQ yield was reduced by almost 50% (w/v). Nitrogen and phosphate toxicity was not only a function of both individual and collective level of ammonium, nitrate and phosphate, but also the level of sucrose and medium strategies. myo inositol was not an absolute requirement in M. elliptica cell suspension culture.

Item Type:

Journal

Additional Information:

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Keywords:

Anthraquiriones, Morinda elliptica, nitrogen, phosphate, myo inosito

Subjects:

Q Science, Computer Science

ID Code:

4142