Production of high frequency multiple shoots and plantlets in six Indian cultivars of cotton (Gossypium hirsutum L.)

Mhatre, A., and Suprasanna, P., and Jaiswal, R., and Kumar, V.V., and Rao, P.S., (1999) Production of high frequency multiple shoots and plantlets in six Indian cultivars of cotton (Gossypium hirsutum L.). Asia Pacific Journal of Molecular Biology & Biotechnology, 7 (1). pp. 73-78.

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Affiliations

Bhabha Atomic Research Centre, Trombay, Mumbal, India. Nuclear Agriculture & Biotechnology Division["lib/metafield:join_corp_creators" not defined]Bhabha Atomic Research Centre, Trombay, Mumbal, India. Nuclear Agriculture & Biotechnology Division["lib/metafield:join_corp_creators" not defined]Bhabha Atomic Research Centre, Trombay, Mumbal, India. Nuclear Agriculture & Biotechnology Division["lib/metafield:join_corp_creators" not defined]Bhabha Atomic Research Centre, Trombay, Mumbal, India. Nuclear Agriculture & Biotechnology Division["lib/metafield:join_corp_creators" not defined]Bhabha Atomic Research Centre, Trombay, Mumbal, India. Nuclear Agriculture & Biotechnology Division

Abstract

A protocol for the production of high frequency multiple shoots using seedling apices with cotyledonary node of six Indian cultivars of cotton (Gossypium hirsutum L.) has been developed. An average of 7 10 shoots per explant were produced directly from the shoot apices excised from 10 day old seedlings on MS medium containing BAP (8.87?M) along with adenine sulphate (81.43 ?M) or kinetin (4.67 ?M). Rooting of isolated shoots was achieved on MS medium containing NAA (0.054?M). Rooted plantlets exhibited 95% survival during hardening in the greenhouse and regenerated plants grew to maturity with normal flowering and boll set.

Item Type:

Journal

Additional Information:

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Keywords:

Cotton, Gossypium hirsutum, plant regeneration, in vitro culture

Subjects:

S Agriculture, Forestry
Q Science, Computer Science

ID Code:

4143

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