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Production of Extra-Cellular Proteases from Marine Bacillus Sp. Cultured in Media Containing Ammonium Sulfate as the Sole Nitrogen Source

Seri Intan, M., and Haslinda H., and Mariatul Fadzillah M., and Ishak M.Y., and Syed Othman S.Z., (2005) Production of Extra-Cellular Proteases from Marine Bacillus Sp. Cultured in Media Containing Ammonium Sulfate as the Sole Nitrogen Source. Malaysian Journal of Microbiology , 1 (2). pp. 30-34. ISSN 9834240708

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Official URL: http://www.usm.my/mjm/issues/vol1no2/research3.pdf

Affiliations

SIRIM Bhd, Environmental and Bioprocess Technology Centre
University Islam Antarabangsa Malaysia, Kulliyah of Engineering
University Islam Antarabangsa Malaysia, Kulliyah of Engineering
SIRIM Bhd, Environmental and Bioprocess Technology Centre
SIRIM Bhd, Environmental and Bioprocess Technology Centre

Abstract

The objective of this study was to evaluate the fermentative characteristics of lactic acid bacteria isolated from local fermented foods and consume raffinose and stachyose during fermentation soymilk. Lactobacillus plantarum pentosus SMN, 01, Lactobacillus casei subsp rhamnosus FNCC, 098, Lactobacillus casei subsp rhamnosus FNCC, 099, Streptococcus thermofilus, 001, Lactobacillus delbrueckii subsp. bulgaricus FNCC, 0045, Lactobacillus plantarum SMN, 25, and Lactobacillus plantarum pentosus FNCC, 235 exhibited variable -galactosidase activity with Lactobacillus plantarum SMN, 25, showing the highest activity in MRS supplemented media. However, all organisms reached the desired therapeutic level (108 cfu/mL) likely due to their ability to metabolize oligosaccharides during fermentation in soymilk at 41 °C. The oligosaccharide metabolism depended on -galactosidase activity. Lactobacillus plantarum SMN, 25, L. plantarum pentosus SMN, 01 and Lactobacillus plantarum pentosus FNCC, 235 reduced raffinose and stachyose by 81.5, 73.0, 67.0%, and 78.0, 72.5, 66.0% respectively in soymilk.

Item Type:Journal
Additional Information:Authors are grateful to the Malaysian Government for providing funds through National Biotechnology Directorate (NBD) Project no. 54801 to carry out this research.
Keywords:Lactobacillus sp, fermentation soymilk, galactosidase, raffinose, stachyose
Subjects:Q Science, Computer Science
ID Code:4975

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