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Optimization of Growth Medium for Efficient Cultivation of Lactobacillus Salivarius i 24 Using Response Surface Method

Lim, C.H., and Rahim, R.A., and Ho, Y.W., and Arbakariya, B.A., (2007) Optimization of Growth Medium for Efficient Cultivation of Lactobacillus Salivarius i 24 Using Response Surface Method. Malaysian Journal of Microbiology , 3 (2). pp. 41-47. ISSN 9834240708

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Official URL: http://www.usm.my/mjm/issues/vol3no2/research8.pdf

Affiliations

Universiti Putra Malaysia, Faculty of Biotechnology and Biomolecular Sciences. Dept. of Bioprocess Technology,
Universiti Putra Malaysia, Faculty of Biotechnology a Universiti Putra Malaysia, Faculty of Biotechnology and Biomolecular Sciences. Dept. of Biomolecular Sciences. Dept. of Cell and Molecular Biology
Universiti Putra Malaysia, Faculty of Biotechnology and Biomolecular Sciences. Dept. of Bioprocess Technology AND Institute of Bioscience. Laboratory of Industrial Biotechnology

Abstract

Production of Lactobacillus salivarius i 24, a probiotic strain for chicken, was studied in batch fermentation using 500 mL Erlenmeyer flask. Response surface method (RSM) was used to optimize the medium for efficient cultivation of the
bacterium. The factors investigated were yeast extract, glucose and initial culture pH. A polynomial regression model with cubic and quartic terms was used for the analysis of the experimental data. Estimated optimal conditions of the factors for growth of L. salivarius i 24 were; 3.32 % (w/v) glucose, 4.31 % (w/v) yeast extract and initial culture pH of 6.10.

Item Type:Journal
Keywords:Lactobacillus salivarius, optimization, response surface method, probiotic for chicken.
Subjects:Q Science, Computer Science
ID Code:5014

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