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Alkaline Phosphatase Activity Assessment Of Two Endodontic Materials: A Preliminary Study

Rajan, S., and Awang H., and Devi, S., and Pooi, A.H., and Hassan H., (2008) Alkaline Phosphatase Activity Assessment Of Two Endodontic Materials: A Preliminary Study. Annals of Dentistry, 15 (1). pp. 5-10. ISSN 0128-7532

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Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=640

Affiliations

University of Malaya. Faculty of Dentistry. Dept. of Children's Dentistry & Orthodontics
University of Malaya. Faculty of Dentistry. Dept. of Children's Dentistry & Orthodontics
University of Malaya. Faculty of Science. Institute of Mathematical Science
University of Malaya. Faculty of Dentistry. Dept. of Medical Microbiology
University of Malaya. Faculty of Science. Institute of Mathematical Science

Abstract

Objective: An in vitro assessment of MG-63 human osteosarcoma cells' alkaline phosphatase (ALP) activity when in contact with calcium hydroxide powder (CH), paste (CHP) and grey mineral trioxide aggregate (MTA). Methods: MG-63 cells were seeded to the three selected materials for durations of 0.25, 0.5, 1, 24, 48 and 72 hours. BCIP-NBT assay was used and ALP activity quantified using ELISA reader at 410 nm. Results: The overall analysis for ALP activity indicated significant interaction between test materials and control (maintenance medium). Subsequently, the test materials were paired and analysed for initial (0.25, 0.5, 1 hour) and delayed response (24, 48 and 72 hours). During the initial response, CH exhibited an increased ALP activity compared to MTA. This interaction was not dependant on duration. The delayed response exhibited elevated ALP activity with CHP when compared to MTA and CH. The interaction of CHP was dependant on duration. Conclusion: All three materials exhibited increased ALP activity.

Item Type:Journal
Additional Information:This research was supported by a grant from University of Malaya, Malaysia (Vote F: F0214/2003A).
Keywords:mineral trioxide aggregate, MTA, calcium hydroxide, alkaline phosphatase, in vitro assessment, MG-63 cell line
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:5037

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