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Antimicrorobial Evaluation Of Sodium Hypochlorite And Ozonated Water On E. Faecalis Biofilm

Ibrahim N,Z,, and Abdullah M. (Mariam Abdullah), (2008) Antimicrorobial Evaluation Of Sodium Hypochlorite And Ozonated Water On E. Faecalis Biofilm. Annals of Dentistry, 15 (1). pp. 20-26. ISSN 0128-7532

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Official URL: http://ejum.fsktm.um.edu.my/ArticleInformation.aspx?ArticleID=642

Affiliations

University of Malaya. Faculty of Dentistry. Dept. of Conservative Dentistry
University of Malaya. Faculty of Dentistry. Dept. of Conservative Dentistry

Abstract

This study aim to evaluate antimicrobial efficacy of sodium hypochlorite (NaOCl) and ozonated water against Enterococci faecalis biofilm. The bacterial biofilm was exposed to 2.62%, 1.31% NaOCl and 0.1 ppm ozonated water over a range of time periods. The presence of viable cells was determined by enumeration of colony forming units (CFU). All experiments were repeated four times (n=4). The effectiveness of the agents was compared using nonparametric Kruskal- Wallis test. The result revealed that 2.62% of NaOCl can completely kill E. faecalis biofilm in 15 minutes whereas 1.31 % NaOCl required a longer time to produce such effect. 0.1 ppm ozonated, however, did not exhibit any antimicrobial effect within the period of time tested. From this study, it can be concluded that 0.1 ppm ozonated water was not comparable with 2.62% and 1.31% NaOCl in antimicrobial efficacy against E. faecalis biofilm.

Item Type:Journal
Keywords:Sodium hypochlorite, ozonated water, biofilm, antimicrobial efficacy
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:5043

1. Kakehashi S, Stanley HR, Fitzgerald RJ, Bethesda. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surgery, Oral Medicine, Oral Pathology 1965; 20: 340-349.

2. Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root filled teeth with apical periodontitis. Int Endo J 1998; 31: 1-7.

3. Chvez de Paz LE, Dahl-n G, Molander A, Moller A, Bergenholtz G. Bacferia recovered from teeth with apical periodontitis after antimicrobial endodontic treatment. Int Endo J 2003; 36: 500-508.

4. Haapasalo M, 0rstavik D. In vitro infection and disinfection of dentinal tubules. J of Dent Res 1987; 66: 1375-1379.

5. Morrison 0, Woodford N, Cooleson B. Enterococci as emerging pathogens of humans. J of Appl Microbiology 1997; 83: 89s-99s.

6. Costerton JW, Cheng KJ, Geesey GG, Ladd TI, Nickel JC, Desgupta M, Martie TJ. Bacterial biofilm in nature and disease. Annals Rev microbiology 1987; 41: 435-464.

7. Shih M, Marshal FJ, Rosen SR. The bactericidal efficiency of sodium hypochlorite as an endodontic irrigant. Oral Surgery, Oral Medicine, Oral Pathology 1970; 29: 613-9.

8. Foley DB, Weine FS, Hagen JC, Deobarrio JJ. Effectiveness of selected irrigants in the elimination of Bacteroides melaninogenicus from the root canal system: An in vitro study. J of Endo 1983; 9: 236-241.

9. Spratt DA, Pratten J, Wilson M, Gulabivala K. An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endo J 2001; 34: 300-307.

10. Abdullah M, Ng Y-L, Gulabivala K, Moles DR, Spratt DA. Susceptibility of two Enterococcus faecalis phenotypes to root canal medications. J of Endo 2005; 31: 30-36.

11. Broadwater WT, Hoehn RC, King PH. Sensitivity of three selected bacterial species to ozone. Appl Microbiology 1973; 26: 391-393.

12. Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J of Endo 2004; 30: 778-781.

13. Heling I, Rotstein I, Dinur T, Szwec-Levine Y, Steinberg D. Bactericidal and cytotoxic effects of sodium hypochlorite and sodium dichloroisocyanurate solutions in vitro. J of Endo 2001; 27: 278-280.

14. Sundqvist G, Figdor 0, Persson S, Sjogren U.Microbiologic analysis of teeth with failed endodontic treatment. Oral Surgery, Oral Medicine, Oral Pathology 1998; 85: 86-93.

15. Moller AJR, Fabricius L, Dahlen G, Othman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J of Dent Res 1981; 89:475-84.

16. Fabricius L, Dahlen G, Ohman AE, Moller AJR. Predominant indigenous oral bacteri'a isolated from infected root canals after varied times of closure. Scand J of Dent Res 1982; 90: 134-144.

17. 0rstavik 0, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol1990; 6:142-149.

18. Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone-generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res 2000; 34: 498-501.

19. Hoigne J, Bader H. The role of hydroxyl radicalreactions in ozonation processes in aqueous solution. Water Res 1976; 10: 377-86.

20. Siqueira JF, De Uzeda M, Fonseca MEF. A scanning electron microscopic evaluation of in vitro dentinal tubules penetration by selected anaerobic bacteria. J of Endo 1996; 22: 308-310.

21. Gulabivala K, Stock CJ, Lewsey JD, Ghori S, Ng,Y.L, Spratt DA. Effectiveness of electrochemically activated water as an irrigant in an infected tooth model. Int Endo J 2004; 37: 624-631.

22. Siqueira JF, Ror;as IN, Favieri A, Lima KC. Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5% and 5.25% sodium hypochloride. J of Endo 2000; 26: 331-334.

23. Berber VB, Gomez BPFA, Sena NT, Vianna ME, Ferraz CCR, Zaia AA, Souza-Filho FJ. Efficacy of various concentrations of NaOCI and instrumentation techniques in reducing Enterococcus faecalis within root canals and dentinal tubules. Int Endo J 2006; 39: 10-17.

24. Racdliffe CE, Potouridou L, Qureshi R, Habahbeh N, Qualtrough A, Worthington H, Drucker DB. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms Actinomyces israelii, A. naeslundii, Candida albicans and Enterococcus faecalis. Int Endo J 2004; 37:438-446.

25. Hems RS, Gulabivala K, Ng Y-L, Ready 0, Spratt DA. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endo J 2005; 38:22- 29.

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