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Evaluation of Polymerase Chain Reaction (PCR) Method and Hybrid Capture II (HCII) Assay for the Detection of Human Papillomavirus in Cervical Scrapings

Saini R., and Shen, T.H., and Othman N.H., and Santhanam, J., and Othman, N., and Tang, T.H., (2007) Evaluation of Polymerase Chain Reaction (PCR) Method and Hybrid Capture II (HCII) Assay for the Detection of Human Papillomavirus in Cervical Scrapings. Medical Journal of Malaysia, 62 (3). pp. 206-209. ISSN 0300-5283

Full text not available from this repository.

Affiliations

Universiti Sains Malaysia. Health Campus. School of Dental Sciences.
Universiti Sains Malaysia. Health Campus. Dept. of Pathology.
Universiti Sains Malaysia. Health Campus. Dept. of Pathology.
Universiti Sains Malaysia. Health Campus. Institute for Research in Molecular Medicine (INFORMM).
Universiti Sains Malaysia. Health Campus. Dept. of Pathology.
Universiti Kebangsaan Malaysia. Institute of Allied Health Sciences. Dept. of Biomedical Sciences.

Abstract

In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8%) in comparison to HCII (36.4%) in detecting HPV although specificity of HCII was much higher (96.6%) than PCR (58.6%). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0%) compared to PCR (42.9%). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.

Item Type:Journal
Additional Information:We would like to acknowledge Prof E.M. deVilliers of DKFZ, Heidelberg, Germany for providing us the clones of HPV 6, 11, 16, 18 that were used as positive controls in this study.
Keywords:PCR, Hybrid Capture II, HPV
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:5630

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