Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

p63 as a Complimentary Basal Cell Specific Marker to High Molecular Weight-Cytokeratin in Distinguishing Prostatic Carcinoma from Benign Prostatic Lesions

Shiran M.S., and Tan, G.C. , and Sabariah A.R., and Rampal, L., and Phang, K.S., (2007) p63 as a Complimentary Basal Cell Specific Marker to High Molecular Weight-Cytokeratin in Distinguishing Prostatic Carcinoma from Benign Prostatic Lesions. Medical Journal of Malaysia, 62 (1). pp. 36-39. ISSN 0300-5283

Full text not available from this repository.

Affiliations

Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Dept. of Pathology.
Hospital Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Pathology.
Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Dept. of Pathology.
Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Dept. of Community Health.
Hospital Universiti Kebangsaan Malaysia. Faculty of Medicine. Dept. of Pathology.

Abstract

The diagnosis of prostatic carcinoma (Pca) on routine biopsies may be challenging, and to date the commonly used marker to distinguish prostate carcinoma from benign prostatic lesions has been High Molecular Weight-Cytokeratin (HMW-CK). However, the antigen of HMW-CK is susceptible to the effect of formalin fixation and causes frequent loss or patchy staining in the obviously benign glands. More recently, antibodies to p63 have been reported to be more sensitive than HMW-CK for the detection of prostatic basal cells. p63, a homologue of tumour suppressor gene p53, is essential for prostate development and is selectively expressed in the nuclei of basal cells of normal prostate glands. The objective of this study is to compare the sensitivity and specificity of HMW-CK and p63 in distinguishing prostatic carcinomas from benign prostatic lesions, as well as determining their positive predictive values. Seventy-two cases from HUKM (comprising 29 prostatic carcinomas and 43 benign prostatic hyperplasias) were stained for both HMW-CK and p63. The sensitivity of p63 and HMW-CK in identifying basal cells in benign glands was 88.37% and 90.70% respectively. The specificity of both reagents was 100%, and the positive predictive value for both reagents was also 100%. Thus, p63 is a useful complementary basal cell specific stain to HMW-CK, and would be very helpful to practicing pathologists in dealing with difficult cases.

Item Type:Journal
Keywords:Prostatic carcinoma, Benign prostatic hyperplasia, Immunomarker, Sensitivity, Specificity, p63, HMW-CK
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:5898

1. Lim GCC, Hatimah Y, Lim TO. The First Report of tile National Cancer Registry Cancer Incidence in Malaysia, 2002.

2. Wu HH, Lapkus O, Corbin M. Comparison of 34βE12 and p63 in 100 consecutive prostate carcinoma diagnosed by needle biopsy. Appl Immunohistochem Mol Morpho 2004; 12: 285-89.

3. Brawer MK, Peehl DM, Stamey TA, Botswick DG. Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 1985; 45: 3663-7.

4. Hedrick L, Epstein JI. Use of keratin 903 as an adjunct in the diagnosis of prostatic carcinoma. Am J Pathol 1989; 13: 389-96.

5. O’Malley FP, Grigon DJ, Shum DT. Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland. Virchows Arch A Pathol Anat Histopathol 1990; 417: 191-6.

6. Parsons JK, Gage WR, Nelson WG, DeMarzo AM. P63 protein expression is rare in prostate adenocarcinoma; implications for cancer diagnosis and carcinogenisis. Urology. 2001; 58: 619-24.

7. Multhaupt HAB, Fessler JN, Warhol MJ. Loss of high molecular weight cytokeratin antigenicity in prostate tissue obtained by transurethral resections. Arch Pathol Lab Med. 2000; 124: 1764-767.

8. Yang XJ, Lecksell K, Gaudin P, Epstein JI. Rare expression of high molecular weight cytokeratin in adenocarcinoma of the prostate gland. Am J Surg Pathol. 1999: 23: 147-52.

9. Oliai BR, Kahane H, Epstein JI. Can basal cell be seen in adenocarcinoma of the prostate? Am J Surgical Pathol. 2002; 26: 1151-160.

10. Yang A, Schweitzer R, Sun D et al. p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature. 1999; 398: 714-8.

11. Signoretti S, Waltregny D, Dilks J et al. p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol. 2000; 157: 1769-75.

12. Varma M, Linden MD, Amin MB. Effect of formalin fixation and epitope retrieval technique on antibody 34βE12 immunostaining of prostate tissue. Mod Pathol. 1999; 12; 472-78.

13. Shah RB, Zhou M, LeBlanc M, Snyder M, Rubin MA. Comparison of the basal cell-specific makers, 34βE12 and p63, a sensitive marker of prostatic cancer. Am J Surg Pathol. 2002; 26: 1161-168.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.