Author, Subjects, Keywords

Cited Author

 
 
   » By Author or Editor
 » Browse Author by Alphabet
 » By Journal
 » By Subjects
 » Malaysian Journals
 » By Type
 » By Year
 » By Latest Additions
 
 
   » By Author
 » Top 20 Authors
 » Top 20 Article
 » Top Journal Cited
 » Top Article Cited
 » Journal Citation Statistics
 » Usage Since Sept 2007


 
 
 

Login | Create Account

Presence of Allelic Loss and PTEN Mutations in Malignant Gliomas from Malay Patients

Zainuddin N., and Jaafar H., and Isa M.N., and Abdullah J.M., (2004) Presence of Allelic Loss and PTEN Mutations in Malignant Gliomas from Malay Patients. Medical Journal of Malaysia, 59 (4). pp. 468-479. ISSN 0300-5283

Full text not available from this repository.

Affiliations

Universiti Sains Malaysia. School of Medical Sciences.Human Genome Centre
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Pathology
International Medical University Malaysia
Universiti Sains Malaysia. School of Medical Sciences. Dept. of Neurosciences.

Abstract

Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to determine the incidence of allelic losses on chromosome 10q, 9p, 17p and 13q and mutations of exons 5, 6 and 8 of the PTEN gene in malignant gliomas. Twelve of 23 (52.2%) malignant glioma cases showed allelic losses whereas 7 of 23 (30.4%) samples showed aberrant band patterns and mutations of the PTEN gene. Four of these cases showed LOH on 10q23 and mutations of the PTEN gene. The data on LOH indicated the involvement of different genes in gliomagenesis whereas mutations of the PTEN gene indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population.

Item Type:Journal
Keywords:Glioma, Loss of heterozygosity, Microsatellite markers, PTEN, Single-stranded conformational polymorphism
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:6137

1. Rasheed BKA, Stenzel TT, McLendon RE, Parsons R. Friedman AH, Friedman HS and Bigner SH. PTEN gene mutations are seen in high grade, but not in low grade gliomas. Cancer Res 1997; 57: 4187-196.

2. Bigner SH, Mark J, Burger PC, Mahaley MS, Bullard DE. Muhlbaier LH and Bigner DD. Specific chromosomal abnormalities in malignant human gliomas. Cancer Res 1988; 48: 405-11.

3. Li J, Yen C, Liaw D et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast and prostate cancer. Science 1997; 275: 1943-947.

4. Watling CJ, van Meyel DJ, Ramsay DA, Macdonald DR and Cairncross JG. Loss of heterozygosity analysis of chromosomes 9, 10 and 17 in gliomas in families. Can J Neurol Sc 1995; 22: 17-21.

5. Lee S, Kim J, Rhee C, Kang Y, Lee J, Hong S and Choi K. Loss of heterozygosity on chromosome 10, 13q (Rb), 17p and p53 gene mutations in human brain gliomas. J Korean Medical Sc 1995; 10: 442-48.

6. Schmidt EE, Ichimura K, Goike HM and Moshref A. Mutational profile of the PTEN gene in primary human astrocytic tumors and cultivated xenografts. J Neuropathol Exp Neurol 1999; 58: 1170 -182.

7. Fujisawa H, Reis RM, Nakamura M, Colella S, Yonekawa Y, Kleihues, P and Ohgaki H. Loss of heterozygosity on chromosome 10 is more extensive in primary (de novo) than in secondary glioblastomas. Lab Invest 2000; 80: 65- 72.

8. Cheng Y, Ng H, Ding M and Zhang S. Molecular analysis of microdissected de novo glioblastomas and paired astrocytic tumors. J Neuropathol Exp Neurol 1999; 58: 120-28.

9. Sonoda Y, Murakami Y, Tominaga T, Kayama T, Yoshimoto T and Sekiya T. Deletion mapping of chromosome 10 in human glioma. Jpn J Cancer Res 1996; 87: 363-67.

10. Hahn M, Wieland I, Koufaki ON, Gorgens H, Sobottka SB, Schackert G and Schackert HK. Genetic alterations of the tumor suppressor gene PTEN/MMAC1 in human brain metastases. Clin Cancer Res 1999; 5: 2431-437.

11. Myers MP, Stolarov JP, Eng C, Li J, Wang SI, Wigler MH, Parsons R and Tonks NK. PTEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase. Proc Natl Acad Sci USA 1997; 94: 9052-57.

12. Sakai A, Thieblemont C, Wellmann A, Jaffe ES and Raffeld M. PTEN gene alterations in lymphoid neoplasms. Blood 1998; 92: 3410-415.

13. Sato N, Tsunoda H, Nishida M, Morishita Y, Takimoto Y, Kubo T and Noguchi M. Loss of heterozygosity O 10q23.3 and mutation of the tumor suppressor gene PTEN in benign endometrial cyst of the ovary: possible sequence progression from benign endometrial cyst to endometroid carcinoma and clear cell carcinoma of the ovary. Cancer Res 2000; 60: 7052-56.

14. Steck PA, Pershouse MA, Jasser SA, Yung WKA, Lin H, Ligon AH, Langford LA, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng DHF and Tavtigian SV. Identification of a candidate tumor suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet 1997; 15: 356-62.

15. Davies MP, Gibbs FE, Halliwell N et al. Mutation in the PTEN MMAC1 gene in archival low grade and high grade gliomas. Br J Cancer 1999; 79: 1542-48.

16. Hill JR, Kuriyama N, Kuriyama H and Israel MA. Molecular genetics of brain tumor. Arch Neurol 1999; 56: 439-42.

17. Maier D, Zhang Z, Taylor E et al. Somatic deletion mapping on chromosome 10 and sequence analysis of PTEN/MMAC1 point to the 10q25-26 region as the primary target in low-grade and high-grade gliomas. Oncogene 1998; 16: 3331-335.

18. Wang SI, Puc J, Li J, Bruce JN, Cairns F, Sidransky D and Parsons R. Somatic mutations of PTEN in glioblastoma multiforme. Cancer Res 1997; 57: 4183-186.

19. Knobbe, C.B., Merlo. A. & Reifenberger, G. PTEN signaling in gliomas. Neuro-oncol. 2002; 4: 196-211.

20. Scarisbrick JJ, Woolford AJ, Russell-Jones R and Whittaker J. Loss of heterozygosity on 10q and microsatellite instability in advanced stages of primary cutaneous T-cell lymphoma and possible association with homozygous deletion of PTEN. Blood 2000; 95: 2937-942.

21. Echt CS, May-Marquardt P, Hseih M and Zahorchak R. Characterization of microsatellite markers in eastern white pine. Genome 1996; 39: 1102-108.

22. Rahman MH, Jaquish B and Khasa PD. Optimization of PCR protocol in microsatellite analysis with silver and SYBR® stains. Plant Mol Biol Reporter 2000; 18: 339-48.

23. Kleihues P, Louis DN, Scheithauer BW and Rorke LB. The WHO classification of tumors of the nervous system/Commentaries. J Neuropathol Exp Neurol 2002; 61: 215- 30.

24. Schmidt EE, Ichimura K, Reifenberger G and Collins VP. CDKN2 (p16/MTSI) gene deletion or CDK4 amplification occurs in the majority of glioblastomas. Cancer Res 1994; 54: 6321-324.

25. Walker DG, Duan W, Popovic EA, Kaye AH, Tomlinson FH and Lavin M. Homozygous deletions of the multiple tumor suppressor gene in the progression of human astrocytomas. Cancer Res 1995; 55: 20-23.

26. Ichimura K, Schmidt EE, Miyakawa A, Goike HM and Collins VP. Distinct patterns of deletion on 10p and 10q suggest involvement of multiple tumor suppressor genes in the development of astrocytic gliomas of different malignancy grades. Genes Chrom Cancer 1995; 22: 9-15.

27. Ali IU, Schriml LM and Dean M: Mutational spectra of PTEN/MMAC1 gene. A tumor suppressor with lipid phosphatase activity. J Natl Cancer Inst 1999; 91: 1922-932.

28. Ishii N, Maier D, Merio A, Tada M, Sawamura Y. Diserens A and Van Meir EG. Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines. Brain Pathol 1999; 9: 469-79.

29. Maher EA, Furnari FB, Bachoo RM, Rowitch DH, Louis DN, Cavenee WK and DePinho RA. Malignant glioma: genetics and biology of a grave matter. Genes Devel 2001; 15: 1311-333.

30. Bostrom J, Cobbers JM, Wolter M et al. Mutation of the PTEN (MMAC1) tumor suppressor gene in a subset of glioblastomas but not in meningiomas with loss of chromosome arm 10q. Cancer Res 1998; 58: 29-33.

31. Kato H, Kato S, Kumabe T et al. Functional evaluation of p53 and PTEN gene mutations in gliomas. Clin Cancer Res 2000; 6: 3937-943.

Repository Staff Only: item control page
 
 
 
 
 
 
© Copyright 2007 MyAIS Faculty of Computer Science and Information Technology, University of Malaya – All Rights Reserved. Malaysian Abstracting and Indexing System is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.