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Differential Gene Expression of Human Adipose-Derived Stem Cells in Osteogenic Induction

Hamid A.A., and Ruszymah B.H.I., and Aminuddin B.S., and Sathappan, S., and Chua, K.H., (2008) Differential Gene Expression of Human Adipose-Derived Stem Cells in Osteogenic Induction. Medical Journal of Malaysia, 63 (Supplement A). pp. 9-10. ISSN 0300-5283

Full text not available from this repository.

Affiliations

UKM Medical Centre. Dept. of Physiology.
UKM Medical Centre. Dept. of Physiology & Tissue Engineering Centre.
Universiti Kebangsaan Malaysia. UKM Medical Centre. Tissue Engineering Centre / Ampang Puteri Specialist Hospital, Malaysia. Ear, Nose & Throat Consultant Clinic.
Subang Jaya Medical Centre, Malaysia.
UKM Medical Centre. Dept. of Physiology & Tissue Engineering Centre.

Abstract

Human adipose-derived stem cells (HADSC) have demonstrated the capacity of differentiating into bone depending on the specific induction stimuli and growth factors. However, investigation on stem cell characteristic after osteogenic differentiation is still lacking. The goal of this study was to investigate the differential expression of stemness and osteogenic genes in non-induced HADSC compared with HADSC after osteogenic induction using quantitative Real Time RT-PCR. Our results showed that OCT-4, REX-1, FZD9, OSC, RUNX, and ALP were up regulated after osteogenic induction. This may indicated that HADSCs after osteogenic induction still possessed some sternness properties.

Item Type:Journal
Additional Information:In conclusion, HADSCs after osteogenic induction still possessed some sternness properties.
Keywords:Stem cells
Subjects:R Medicine, Dentistry, Pharmacy, Nursing
ID Code:6483

1. Zuk PA, Zhu M, Ashjian P et al. Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell. 2002; 13: 4279-95.

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